| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,074,177 | G→A | 29.8% | V168I (GTC→ATC) | flu → | antigen 43 (Ag43) phase‑variable biofilm formation autotransporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,074,177 | 0 | G | A | 29.8% | 69.3 / 34.1 | 47 | V168I (GTC→ATC) | flu | antigen 43 (Ag43) phase‑variable biofilm formation autotransporter |
| Reads supporting (aligned to +/- strand): ref base G (17/16); new base A (2/12); total (19/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAACACCACGCTGAATGGTGGCGAACAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGATAAGGGCTGGCAGGTCGTCAAGCCCGGTACAGTGGCAACGGATACCGTTGTTAAT > W3110S.gb/2074115‑2074242 | tgAACACCACGCTGAATGGTGGCGAACAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCGTCAtt > 1:673779/1‑68 (MQ=255)tgAACACCACGCTGAATGGTGGCGAACAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTa > 1:2634748/1‑69 (MQ=255) aaCACCACGCTGAATGGTGGCGAACAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTa < 1:865329/67‑1 (MQ=255) aaCACCACGCTGAATGGTGGCGAACAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTa < 1:305365/67‑1 (MQ=255) caccacGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGa < 1:1181116/69‑1 (MQ=255) caccacGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGa < 1:2743842/69‑1 (MQ=255) cTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGAt < 1:1034058/63‑1 (MQ=255) cTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGAt < 1:30815/63‑1 (MQ=255) tGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGAt < 1:2445838/62‑1 (MQ=255) tGAATGGTGGCGAACAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGATAAGGGCt < 1:893886/69‑1 (MQ=255) tGAATGGTGGCGAACAGTGGATGCATGAGGCGGCGATAGCCACAGGAACCGTCATTAATGATAAGGGCt < 1:2853933/69‑1 (MQ=255) tggtggCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGATAAGGGCTGGc < 1:1342576/68‑1 (MQ=255) tggtggCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGATAAGGGCTGGc < 1:1511137/68‑1 (MQ=255) tggtggCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGATAAGGGCTGGc < 1:1767146/68‑1 (MQ=255) gTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAAt < 1:930430/43‑1 (MQ=255) gTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGATAAGGGCTGGCAGGTCGTCAAGcc > 1:869765/1‑69 (MQ=255) gTGGATGCATGAGGGGGCGATAGCCACAGGAACCAACATTAATGATAAGGGCTGGAAGGTCGTCAAGcc > 1:2396549/1‑69 (MQ=255) tGGATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGAt < 1:3217395/45‑1 (MQ=255) tGGATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGAt < 1:3137086/45‑1 (MQ=255) tGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAAt < 1:1951734/42‑1 (MQ=255) gATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGAt > 1:525441/1‑43 (MQ=255) gATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGAt > 1:2564770/1‑43 (MQ=255) gATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGAt > 1:330102/1‑43 (MQ=255) gATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGAt > 1:3072372/1‑43 (MQ=255) gATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGAt > 1:2826059/1‑43 (MQ=255) gATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGAt > 1:2657681/1‑43 (MQ=255) gATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGATAAGGGCTGGCAGGTCGTCAAGCCCgg > 1:3045492/1‑69 (MQ=255) aGGGGGCGATAGCCACAGGAACCGTCATTAATGAt > 1:2952784/1‑35 (MQ=255) aGGGGGCGATAGCCACAGGAACCGTCATTAATGAt < 1:1865999/35‑1 (MQ=255) aGGGGGCGATAGCCACAGGAACCGTCATTAATGAt > 1:2898928/1‑35 (MQ=255) aGGGGGCGATAGCCACAGGAACCGTCATTAATGAt < 1:1893282/35‑1 (MQ=255) aGGGGGCGATAGCCACAGGAACCGTCATTAATGAt > 1:1850234/1‑35 (MQ=255) aGGGGGCGATAGCCACAGGAACCGTCATTAATGATAAGGGCTGGCAg < 1:2649341/47‑1 (MQ=255) aGGGGGCGATAGCCACAGGAACCGTCATTAATGATAAGGGCTGGCAg < 1:797890/47‑1 (MQ=255) aGGGGGCGATAGCCACAGGAACCGTCATTAATGATAAGGGCTGGCAg < 1:3499587/47‑1 (MQ=255) aGGGGGCGATAGCCACAGGAACCGTCATTAATGATAAGGGCTGGCAg < 1:1276669/47‑1 (MQ=255) aGGGGGCGATAGCCACAGGAACCGTCATTAAAGAt > 1:368468/1‑35 (MQ=255) gggggCGATAGCCACAGGAACCGTCATTAATGATAAGGGCTGGCAg > 1:3300866/1‑46 (MQ=255) gggggCGATAGCCACAGGAACCGTCATTAATGATAAGGGCTGGCAg > 1:3166181/1‑46 (MQ=255) aGCCACAGGAACCATCATTAATGATAAGGGCTGGc < 1:1350666/35‑1 (MQ=255) acaGGAACCGTCATTAATGATAAGGGCTGGCAGGTCGTCAAGcc > 1:249966/1‑44 (MQ=255) aGGAACCATCATTAATGATAAGGGCTGGCAGGTCGTCAAGCCCGGTACAGTGGCAACGGATACCgttg < 1:1901737/68‑1 (MQ=255) ggAACCGTCATTAATGATAAGGGCTGGCAGGTCGTCAAGCCCGGTACAGTGGCAACGGATACCgttgtt < 1:1429215/69‑1 (MQ=255) ggAACCGTCATTAATGATAAGGGCTGGCAGGTCGTCAAGCCCGGTACAGTGGCAACGGATACCgttgtt < 1:385023/69‑1 (MQ=255) ggAACCGTCATTAATGATAAGGGCTGGCAGGTCGTCAAGCCCGGTACAGTGGCAACGGATACCgttgtt < 1:1121049/69‑1 (MQ=255) aaCCGTCATTAATGATAAGGGCTGGCAGGTCGTCAAGCCCGGTAc < 1:3189011/45‑1 (MQ=255) aCCGTCATTAATGATAAGGGCTGGCAGGTCGTCAAGCCCGGTACAGTGGCAACGGATACCGTTGTTAAt > 1:3067409/1‑69 (MQ=255) | TGAACACCACGCTGAATGGTGGCGAACAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGATAAGGGCTGGCAGGTCGTCAAGCCCGGTACAGTGGCAACGGATACCGTTGTTAAT > W3110S.gb/2074115‑2074242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |