| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,077,980 | C→T | 69.2% | Y355Y (TAC→TAT) | yeeR → | predicted membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,077,980 | 0 | C | T | 69.2% | 29.4 / 19.3 | 26 | Y355Y (TAC→TAT) | yeeR | predicted membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (5/3); new base T (9/9); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGC > W3110S.gb/2077916‑2078046 | cctcctGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGct > 1:291895/1‑69 (MQ=255)cctcctGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGct > 1:928023/1‑69 (MQ=255) tcctGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGctc < 1:346830/68‑1 (MQ=255) aCAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:3315660/1‑68 (MQ=255) aGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga > 1:2343458/1‑66 (MQ=255) aCATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCgaaacaga < 1:1193003/69‑1 (MQ=255) aCATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCgaaacaga < 1:2213759/69‑1 (MQ=255) aCATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCgaaacaga < 1:542930/69‑1 (MQ=255) atGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAacac > 1:2776941/1‑69 (MQ=255) tGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:2473229/65‑1 (MQ=255) ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcgcg < 1:197019/54‑1 (MQ=255) ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcgcg < 1:2486312/54‑1 (MQ=255) ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcgcg < 1:112504/54‑1 (MQ=255) ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcgcg < 1:301179/54‑1 (MQ=255) aaaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAacac > 1:2704656/1‑59 (MQ=255) tCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:697145/51‑1 (MQ=255) tCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:1841521/51‑1 (MQ=255) tCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga > 1:2985731/1‑45 (MQ=255) aGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATg < 1:450297/69‑1 (MQ=255) aGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATg > 1:2163914/1‑69 (MQ=255) ccGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:3608680/1‑69 (MQ=255) ccGGAATGGTATTCCTCCTCTCTGTGTCGCgaaaca > 1:40484/1‑36 (MQ=25) ccGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:2245211/1‑69 (MQ=255) tACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGc > 1:3199168/1‑69 (MQ=255) tACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGc > 1:670300/1‑69 (MQ=255) tACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGc > 1:1858449/1‑69 (MQ=255) | CCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGC > W3110S.gb/2077916‑2078046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |