| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,096,084 | G→A | 19.3% | E160E (GAG→GAA) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,096,084 | 0 | G | A | 19.3% | 144.0 / 25.8 | 62 | E160E (GAG→GAA) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (28/22); new base A (4/8); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
AACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTC > W3110S.gb/2096022‑2096151 | aaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTc > 1:1428483/1‑69 (MQ=255) cATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTc > 1:840621/1‑67 (MQ=255) ggCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCa > 1:2329508/1‑59 (MQ=255) ggCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCa < 1:3150238/59‑1 (MQ=255) ggCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCa < 1:3548191/59‑1 (MQ=255) ggCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCa > 1:162325/1‑59 (MQ=255) ggCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCa > 1:3074087/1‑59 (MQ=255) gCATTACAGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCa < 1:120952/58‑1 (MQ=255) ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTg < 1:2340598/69‑1 (MQ=255) ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTg < 1:2150550/69‑1 (MQ=255) ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTg < 1:1833494/69‑1 (MQ=255) ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTg < 1:988695/69‑1 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAAGTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:3706655/1‑69 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGAc > 1:2699003/1‑64 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGt < 1:1195270/66‑1 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGt < 1:1881253/66‑1 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGt < 1:898729/66‑1 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:3018019/1‑69 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:2534256/1‑69 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:513269/1‑69 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:2482867/1‑69 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:3398134/1‑69 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:750810/1‑69 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:1539838/1‑69 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:1264304/1‑69 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc > 1:1114117/1‑69 (MQ=255) ggTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:665283/69‑1 (MQ=255) ggTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:2510171/69‑1 (MQ=255) tttACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTa > 1:2464941/1‑69 (MQ=255) tttACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTa > 1:1684635/1‑69 (MQ=255) cGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCa > 1:2056693/1‑52 (MQ=255) cGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCa > 1:371516/1‑52 (MQ=255) cGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCa > 1:78331/1‑52 (MQ=255) cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTc < 1:618780/68‑1 (MQ=255) cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTc < 1:2729720/68‑1 (MQ=255) cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTc < 1:1043066/68‑1 (MQ=255) tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTg < 1:1826235/55‑1 (MQ=255) aCGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCa > 1:2937874/1‑67 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACAAGACGTGACCGTTACGCTCACGt < 1:802081/67‑1 (MQ=255) aaaCCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:3671714/50‑1 (MQ=255) aaaCCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTa < 1:3419742/69‑1 (MQ=255) aaaCCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTa < 1:1165833/69‑1 (MQ=255) aaaCCCTGAACTGGCCAATGATTGGCGAACAACTCACAAGACGTGACCGTTACGCTCACGTAGTGCGTa < 1:2257093/69‑1 (MQ=255) aCCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGt < 1:2675000/42‑1 (MQ=255) cccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAAt > 1:1083151/1‑68 (MQ=255) cccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAAt > 1:1107233/1‑68 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga < 1:3113369/69‑1 (MQ=255) tGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga < 1:1894835/68‑1 (MQ=255) aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGt < 1:849618/45‑1 (MQ=255) aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGt < 1:3515822/45‑1 (MQ=255) aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGt < 1:2462610/45‑1 (MQ=255) aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGt < 1:796170/45‑1 (MQ=255) aTGATTGGCGAACAACTCACCAGACGTGACCGTTACTCTCACGTAGTGCGTa < 1:311555/52‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGa > 1:3661787/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGa > 1:762286/1‑68 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3359848/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3303430/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3355525/1‑69 (MQ=255) aTTGGCGAGCAACTCACAAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:606582/1‑69 (MQ=255) aTTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:749460/1‑69 (MQ=255) tgtcGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1192350/4‑69 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:248442/69‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTTACGTTc < 1:474536/68‑1 (MQ=255) | AACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTC > W3110S.gb/2096022‑2096151 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |