| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,129,117 | C→T | 40.0% | A71T (GCC→ACC) | fcl ← | bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase and GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,129,117 | 0 | C | T | 40.0% | 13.9 / 25.3 | 25 | A71T (GCC→ACC) | fcl | bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase and GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase |
| Reads supporting (aligned to +/- strand): ref base C (4/11); new base T (3/7); total (7/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01 | |||||||||||
GTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAA > W3110S.gb/2129052‑2129180 | gTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGtaac < 1:759125/69‑1 (MQ=255)gTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGtaac < 1:1836921/69‑1 (MQ=255)gTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGtaac < 1:1956605/69‑1 (MQ=255)gTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGtaac < 1:3216975/69‑1 (MQ=255)gTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGtaac < 1:2863084/69‑1 (MQ=255) ttGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGtaac < 1:2948104/59‑1 (MQ=255) ttGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGTAACAATGCCGccc > 1:1249908/1‑69 (MQ=255) tGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGtaaca < 1:3244812/59‑1 (MQ=255) gCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGtaac < 1:7592/57‑1 (MQ=255) cAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGTAACAATGCCGCCCACTTTcg > 1:3433213/1‑69 (MQ=255) aaTCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTcgc > 1:1704390/1‑69 (MQ=255) aaTCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑AGTTGTTGGCAACAATGCCGCCCACTTTcgc > 1:594403/1‑69 (MQ=255) tcatcaTGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTcgc < 1:824526/67‑1 (MQ=255) tcatcaTGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTcgc < 1:3318131/67‑1 (MQ=255) catcatGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGcaac < 1:1280952/48‑1 (MQ=255) catcatGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGcaac < 1:3488772/48‑1 (MQ=255) gTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCa < 1:1697923/68‑1 (MQ=255) gTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCa < 1:3230505/68‑1 (MQ=255) gATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCa < 1:1517241/59‑1 (MQ=255) gATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCa < 1:237027/59‑1 (MQ=255) gATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCa > 1:2455243/1‑59 (MQ=255) gAAATCCGCCGGATAGG‑‑‑TGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTc < 1:3232522/69‑1 (MQ=255) aTCCGCCGGATAGGTGTTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTc < 1:2929853/69‑1 (MQ=255) aTCCGCCGGATAGG‑‑‑TGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCaa < 1:3182488/68‑1 (MQ=255) tCCGCCGGATAGG‑‑‑TGTTGTTGGTAACAATGCCTCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATa < 1:1592089/69‑1 (MQ=255) gccgGATAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACttt < 1:3021374/38‑1 (MQ=255) ccgGATAGG‑‑‑TGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGtt < 1:3224052/69‑1 (MQ=255) tAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTcgccgccgc < 1:309146/41‑1 (MQ=255) tAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTcgccgccgc < 1:2127516/41‑1 (MQ=255) tAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTcgccgccgc < 1:2677658/41‑1 (MQ=255) tAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTcgccgccgc < 1:3538098/41‑1 (MQ=255) tAGG‑‑‑TGTTGTTGGCAACAAAGCCGCCCACTTTcgccgccgc < 1:2685267/41‑1 (MQ=255) gttgGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAg < 1:2152779/40‑1 (MQ=255) gttgGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGaa > 1:1428098/1‑69 (MQ=255) gttgGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATAc > 1:12822/1‑53 (MQ=255) | GTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGG‑‑‑TGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAA > W3110S.gb/2129052‑2129180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |