| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,157,782 | T→C | 88.3% | L128S (TTG→TCG) | mdtB → | multidrug efflux system, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,157,782 | 0 | T | C | 88.3% | 171.7 / 11.7 | 60 | L128S (TTG→TCG) | mdtB | multidrug efflux system, subunit B |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base C (19/34); total (21/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
AACATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCATGA > W3110S.gb/2157717‑2157847 | aaCATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACttgttg > 1:832310/1‑67 (MQ=255)aaCATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACttgttg > 1:1212912/1‑67 (MQ=255) ttACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCg > 1:2122896/1‑66 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGGAGTGCAGGGCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCg < 1:2704938/68‑1 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGa < 1:380578/69‑1 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGa < 1:2299969/69‑1 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCg < 1:2899314/68‑1 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCg < 1:2399863/68‑1 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGa < 1:2889397/69‑1 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGa < 1:2783799/69‑1 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGa < 1:3052264/69‑1 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGa < 1:2520234/69‑1 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGa > 1:3603214/1‑69 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGa < 1:1970383/69‑1 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGa > 1:1866528/1‑69 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGa < 1:586612/69‑1 (MQ=255) aCCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGa < 1:889806/69‑1 (MQ=255) cTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTg > 1:3585056/1‑69 (MQ=255) tggcGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTa < 1:2970924/65‑1 (MQ=255) tGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTa < 1:41577/68‑1 (MQ=255) tGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTa < 1:1486383/68‑1 (MQ=255) tGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTa < 1:1338709/68‑1 (MQ=255) gTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAAc > 1:435364/1‑69 (MQ=255) gTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAAc > 1:2652996/1‑69 (MQ=255) gTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAAc > 1:2789907/1‑69 (MQ=255) gTCGCCGAGCAGGAAGTGCAGGCAGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAAc > 1:274761/1‑69 (MQ=255) tCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTaa < 1:2974405/67‑1 (MQ=255) tCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTaa < 1:2978703/67‑1 (MQ=255) tCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTaa < 1:3522249/67‑1 (MQ=255) gCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCaa < 1:3022541/69‑1 (MQ=255) cAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCa > 1:728378/1‑67 (MQ=255) cAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCa < 1:606176/67‑1 (MQ=255) cAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCa < 1:3495893/67‑1 (MQ=255) cAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCa < 1:3285331/67‑1 (MQ=255) ggCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGt < 1:1972020/69‑1 (MQ=255) ggCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGt < 1:2324519/69‑1 (MQ=255) cgcgATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGaa < 1:3335024/69‑1 (MQ=255) cgcgATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGcc > 1:62642/1‑42 (MQ=255) cgcgATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGaa < 1:2987932/69‑1 (MQ=255) aTTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAAccc > 1:3099295/1‑68 (MQ=255) aTTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAAccc > 1:1833428/1‑68 (MQ=255) ttAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAAccc > 1:3574161/1‑67 (MQ=255) ttAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAAccc > 1:1993505/1‑67 (MQ=255) ttAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAAccc > 1:3717664/1‑67 (MQ=255) tAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAAccc > 1:1691519/1‑66 (MQ=255) aCGCTGCGACCAACTTGTCTCCGAGCGATCTGCCTAAcc > 1:1596072/1‑39 (MQ=38) aCGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGa > 1:324914/1‑60 (MQ=255) aCGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGa > 1:3692472/1‑60 (MQ=255) aCCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATccgccg > 1:1412484/1‑69 (MQ=255) aCCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATccgccg > 1:1518981/1‑69 (MQ=255) cAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGAt < 1:3247254/69‑1 (MQ=255) cAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGAt < 1:2660922/69‑1 (MQ=255) cAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGAt < 1:490655/69‑1 (MQ=255) cAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGAt < 1:3109686/69‑1 (MQ=255) cAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGAt < 1:3499323/69‑1 (MQ=255) cAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGAt < 1:3159390/69‑1 (MQ=255) cAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGAt < 1:3217509/69‑1 (MQ=255) aCTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGAt < 1:2595057/67‑1 (MQ=255) aCTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGAt < 1:1219644/67‑1 (MQ=255) cTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCAt < 1:395442/69‑1 (MQ=255) cTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCAt < 1:573528/69‑1 (MQ=255) cTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCAt < 1:750759/69‑1 (MQ=255) ttgtcgCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGACCCGCCGATCATg < 1:2809438/69‑1 (MQ=255) tgttgCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCATGa > 1:369772/1‑69 (MQ=255) | AACATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCATGA > W3110S.gb/2157717‑2157847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |