| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,160,543 | C→T | 71.4% | F7F (TTC→TTT) | mdtC → | multidrug efflux system, subunit C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,160,543 | 0 | C | T | 71.4% | 36.1 / 19.0 | 28 | F7F (TTC→TTT) | mdtC | multidrug efflux system, subunit C |
| Reads supporting (aligned to +/- strand): ref base C (5/3); new base T (6/14); total (11/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
TGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCATACTGGGC > W3110S.gb/2160479‑2160606 | tgtgGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCAttt < 1:3385806/69‑1 (MQ=255) cAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGccc < 1:471890/69‑1 (MQ=255) aaaaGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGccc < 1:1857342/68‑1 (MQ=255) aaaGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGcc > 1:2309801/1‑66 (MQ=255) ccGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGc < 1:1204487/69‑1 (MQ=255) ccGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGc < 1:3686135/69‑1 (MQ=255) cccGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGAt < 1:550132/67‑1 (MQ=255) gTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGAtttt < 1:1237322/67‑1 (MQ=255) ggaggCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc < 1:2013432/69‑1 (MQ=255) ggaggCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc < 1:2600038/69‑1 (MQ=255) ggaggCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc < 1:2280309/69‑1 (MQ=255) gaggCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGcgacga > 1:1538208/1‑52 (MQ=255) gaggCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGcgacga > 1:1145441/1‑52 (MQ=255) gaggCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGcgacga > 1:2964477/1‑52 (MQ=255) aaGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTAccc > 1:3034895/1‑69 (MQ=255) aGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCAt < 1:1846217/63‑1 (MQ=255) aGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCAt < 1:299268/63‑1 (MQ=255) aGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGAttt > 1:1215012/1‑47 (MQ=255) gTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCgg > 1:3700148/1‑69 (MQ=255) ttttttGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTc > 1:2669263/1‑47 (MQ=255) ttttGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCAt < 1:2457659/69‑1 (MQ=255) tttGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCAt < 1:2215369/68‑1 (MQ=255) ttGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCATa < 1:1904392/68‑1 (MQ=255) gCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTa < 1:1823062/52‑1 (MQ=255) ctctTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCATACTGGGc > 1:3049273/1‑69 (MQ=255) tctTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc < 1:1325480/43‑1 (MQ=255) ttCATTTACCGCCCGGTGGCGACGATTTTACTGTCg > 1:1868111/1‑36 (MQ=255) ttCATTTACCGCCCGGTGGCGACGATTTTACTGTCg > 1:605480/1‑36 (MQ=255) | TGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCATACTGGGC > W3110S.gb/2160479‑2160606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |