| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,172,235 | C→T | 11.6% | pseudogene (42/447 nt) pseudogene (1636/2041 nt) |
gatR ← gatR ← |
DNA‑binding transcriptional regulator, N‑ter fragment; ECK2083:JW5340:b2087 DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,172,235 | 0 | C | T | 11.6% | 119.8 / 6.7 | 43 | pseudogene (42/447 nt) pseudogene (1636/2041 nt) | gatR gatR | DNA‑binding transcriptional regulator, N‑ter fragment; ECK2083:JW5340:b2087 DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498 |
| Reads supporting (aligned to +/- strand): ref base C (17/21); new base T (3/2); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01 | |||||||||||
CCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATA > W3110S.gb/2172174‑2172301 | ccAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGatat < 1:666241/69‑1 (MQ=255) gagTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATAtttt > 1:2292913/1‑69 (MQ=255) gagTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATAtttt > 1:841533/1‑69 (MQ=255) gagTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATAtttt > 1:2623844/1‑69 (MQ=255) gagTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCAGGGAGACTGTTGGTAATCAcc > 1:370608/1‑59 (MQ=255) aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGc > 1:447221/1‑68 (MQ=255) aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCg > 1:3550510/1‑69 (MQ=255) tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTc > 1:2801941/1‑69 (MQ=255) tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTc > 1:1429902/1‑69 (MQ=255) tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTc > 1:151451/1‑69 (MQ=255) tttATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCg > 1:185324/1‑50 (MQ=255) tATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCg > 1:510004/1‑48 (MQ=255) tATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGt > 1:1676616/1‑64 (MQ=255) ttttCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTa < 1:1442049/67‑1 (MQ=255) aaGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGc > 1:3609468/1‑69 (MQ=255) ggCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCAtt > 1:527870/1‑55 (MQ=255) ggCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCAtt > 1:2456005/1‑55 (MQ=255) ggCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt < 1:3106148/69‑1 (MQ=255) ggCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt < 1:986542/69‑1 (MQ=255) ggCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt < 1:2363107/69‑1 (MQ=255) ggCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt < 1:3655789/69‑1 (MQ=255) ggCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt < 1:74780/69‑1 (MQ=255) aaaCGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAg < 1:1477331/69‑1 (MQ=255) aaaCGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGGCATTAATCCTTCGGCGATAAg < 1:2904679/69‑1 (MQ=255) aaCGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc < 1:2475125/69‑1 (MQ=255) aaCGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc < 1:82192/69‑1 (MQ=255) aCGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTa < 1:2514004/51‑1 (MQ=255) aCGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCa < 1:1989600/69‑1 (MQ=255) cGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCAt < 1:1831291/69‑1 (MQ=255) cGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCAt < 1:11979/69‑1 (MQ=255) tGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGt < 1:1976974/69‑1 (MQ=255) gCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGt < 1:2109069/41‑1 (MQ=255) gggAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCg < 1:1111930/67‑1 (MQ=255) agaCTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACtt > 1:2884578/1‑69 (MQ=255) ttttGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt < 1:1079687/47‑1 (MQ=255) tGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt < 1:1795081/49‑1 (MQ=255) tGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt < 1:575764/49‑1 (MQ=255) tGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt < 1:609362/49‑1 (MQ=255) cACCGTGATATTTTTGGCGGTCATTAATCCTTCGGc > 1:1038400/1‑36 (MQ=255) cACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCg > 1:1480617/1‑50 (MQ=255) aCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAAt < 1:3391801/68‑1 (MQ=255) aCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATa > 1:180841/1‑69 (MQ=255) aCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATa > 1:1760155/1‑69 (MQ=255) aCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATa > 1:2721663/1‑69 (MQ=255) | CCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATA > W3110S.gb/2172174‑2172301 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |