| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,264,229 | C→T | 28.3% | L172L (CTG→CTA) | fruA ← | fused fructose‑specific PTS enzyme IIB'BC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,264,229 | 0 | C | T | 28.3% | 85.1 / 36.7 | 53 | L172L (CTG→CTA) | fruA | fused fructose‑specific PTS enzyme IIB'BC components |
| Reads supporting (aligned to +/- strand): ref base C (20/18); new base T (9/6); total (29/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
TGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGT > W3110S.gb/2264164‑2264291 | tGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACTATACATCGGTTTACCAGCAAATTTCGCTAg < 1:504509/68‑1 (MQ=255) cgGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATc < 1:3313342/67‑1 (MQ=255) cgGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATc < 1:1253396/67‑1 (MQ=255) ttttCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACt < 1:1230023/68‑1 (MQ=255) tttCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTc > 1:3541376/1‑69 (MQ=255) ttcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAg > 1:118838/1‑59 (MQ=255) ttcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAg > 1:3597383/1‑59 (MQ=255) ttcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAg > 1:2128426/1‑59 (MQ=255) ttcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAg > 1:3722890/1‑59 (MQ=255) ttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGat < 1:740843/68‑1 (MQ=255) cAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTGGATATCTgccg < 1:964632/68‑1 (MQ=255) ggTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGata > 1:406797/1‑55 (MQ=255) ggTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGata < 1:2374017/55‑1 (MQ=255) ggTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGata > 1:1048026/1‑55 (MQ=255) tAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATc > 1:320646/1‑68 (MQ=255) tAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATc > 1:288984/1‑68 (MQ=255) ggTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATc > 1:1243971/1‑64 (MQ=255) ggTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATc > 1:3430980/1‑64 (MQ=255) ggTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATc > 1:3212394/1‑64 (MQ=255) aCGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATc > 1:2291587/1‑48 (MQ=255) aCGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATc > 1:1112475/1‑69 (MQ=255) aCGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATc > 1:3126288/1‑69 (MQ=255) cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATcc > 1:120285/1‑69 (MQ=255) cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATcc > 1:809541/1‑69 (MQ=255) cGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATcc < 1:1040790/69‑1 (MQ=255) aTACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgc > 1:496929/1‑69 (MQ=255) aTCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCcac < 1:53037/50‑1 (MQ=255) aTCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCcac < 1:432736/50‑1 (MQ=255) aTCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCcac < 1:726426/50‑1 (MQ=255) aTCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCcac < 1:2465943/50‑1 (MQ=255) aTCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCcac < 1:249172/50‑1 (MQ=255) tCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgctgct > 1:3365248/1‑68 (MQ=255) cGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCtgctgc < 1:512391/69‑1 (MQ=255) ggTTTACCAGCAAATTTCGCTAGATCCACTTTGata > 1:2269579/1‑36 (MQ=37) ggTTTACCAGCAAATTTCGCTAGATCCACTTGGata > 1:633051/1‑36 (MQ=37) ggTTTACCAGCAAATTTCGCTAGATCCACTTCGata > 1:2737634/1‑36 (MQ=255) ggTTTACCAGCAAATTTCGCTAGATCCACTTCGata > 1:3644739/1‑36 (MQ=255) ggTTTACCAGCAAATTTCGCTAGATCCACTTCGata > 1:1084387/1‑36 (MQ=255) ggTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAg > 1:2597912/1‑56 (MQ=255) ggTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAg > 1:385826/1‑56 (MQ=255) ggTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCtgctgc < 1:1029983/68‑1 (MQ=255) ttACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAct < 1:2065716/69‑1 (MQ=255) ttACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAct < 1:1249165/69‑1 (MQ=255) aGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGActtctt < 1:3050423/68‑1 (MQ=255) tttCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGt < 1:1256536/69‑1 (MQ=255) tttCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGt < 1:2901592/69‑1 (MQ=255) tttCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGt < 1:3072822/69‑1 (MQ=255) ttCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAct < 1:578421/57‑1 (MQ=255) ttCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAct < 1:1432226/57‑1 (MQ=255) ttCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAct > 1:287002/1‑57 (MQ=255) ttCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAct < 1:81341/57‑1 (MQ=255) ttCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAct > 1:894621/1‑57 (MQ=255) ttCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAct > 1:3000252/1‑57 (MQ=255) | TGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGT > W3110S.gb/2264164‑2264291 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |