| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,295,693 | C→T | 22.2% | A18T (GCG→ACG) | ccmH ← | heme lyase, CcmH subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,295,693 | 0 | C | T | 22.2% | 74.3 / 14.6 | 36 | A18T (GCG→ACG) | ccmH | heme lyase, CcmH subunit |
| Reads supporting (aligned to +/- strand): ref base C (16/12); new base T (4/4); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCTTCAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCGGCCTCCTT > W3110S.gb/2295625‑2295758 | ttcttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGc < 1:318095/69‑1 (MQ=255)ttcttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGc < 1:2770343/69‑1 (MQ=255) cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:2648637/1‑69 (MQ=255) cAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAgcg > 1:3163609/1‑69 (MQ=255) gAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGa < 1:1601982/69‑1 (MQ=255) aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGatca < 1:950988/69‑1 (MQ=255) gttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGatcatc > 1:1019865/1‑68 (MQ=255) gttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGatcatc > 1:1715012/1‑68 (MQ=255) gttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcag < 1:2689098/67‑1 (MQ=255) gttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcag < 1:2689096/67‑1 (MQ=255) gttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATcatcag < 1:2180984/67‑1 (MQ=255) gttCCTGTGCTTCATCTTTAAACCGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcag < 1:2700912/67‑1 (MQ=255) ccTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATcatcagcatc < 1:1009444/68‑1 (MQ=255) tgCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCAc > 1:1493497/1‑69 (MQ=255) tgCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCAc > 1:2541602/1‑69 (MQ=255) tgCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCAc > 1:1387904/1‑69 (MQ=255) tttAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATa > 1:2062633/1‑68 (MQ=255) tttAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATcatcagc > 1:813929/1‑52 (MQ=255) tAAACTGCAACACATCGATGGTCGCCAGCGCTGAGc < 1:2933604/36‑1 (MQ=255) tAAACTGCAACACATCGATGGTCGCCAGCGCTGAGc < 1:2270221/36‑1 (MQ=255) tAAACTGCAACACATCGATGGTCGCCAGCGCTGAGc < 1:2265881/36‑1 (MQ=255) cTGCAAGACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCt > 1:8348/1‑69 (MQ=255) cTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCt < 1:470861/69‑1 (MQ=255) cTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAAc < 1:2617415/67‑1 (MQ=255) cTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCt > 1:130729/1‑69 (MQ=255) cTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCt > 1:2193733/1‑69 (MQ=255) cTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCt > 1:53923/1‑69 (MQ=255) cTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCt > 1:1416440/1‑69 (MQ=255) tGCAACACATCGATGGTCGCCAGCGCTGAGGCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTc > 1:2230463/1‑69 (MQ=255) acacATCGATGGTCGCCAGCGCTGAGCCGGAGatca < 1:3275142/36‑1 (MQ=255) aTCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCg < 1:3461729/69‑1 (MQ=255) tCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag > 1:3581453/1‑41 (MQ=255) gATGGTCGCCAGCGCTGAGCCGGAGATcatcagcatcat < 1:2077893/39‑2 (MQ=255) gATGGTCGCCAGCGCTGAGCCGGAGATcatcagcatcag < 1:1200925/39‑1 (MQ=255) gATGGTCGCCAGCGCTGAGCCGGAGATcatcaacatcag > 1:3429258/1‑39 (MQ=255) tGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCGGcctc > 1:286797/1‑69 (MQ=255) tGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCGGcctc > 1:1661827/1‑69 (MQ=255) tCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCGGCCTCCtt > 1:370674/1‑69 (MQ=255) | TTCTTCAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCGGCCTCCTT > W3110S.gb/2295625‑2295758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |