| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,307,310 | C→T | 33.3% | V24V (GTC→GTT) | eco → | ecotin, a serine protease inhibitor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,307,310 | 0 | C | T | 33.3% | 23.2 / 13.6 | 21 | V24V (GTC→GTT) | eco | ecotin, a serine protease inhibitor |
| Reads supporting (aligned to +/- strand): ref base C (5/9); new base T (5/2); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATTCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGATTCA > W3110S.gb/2307246‑2307375 | ccATTCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGc < 1:3463148/69‑1 (MQ=255) aTTGTTTGCCGCTTTCGCTACCACTTCCGTCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATcgc > 1:2640628/1‑69 (MQ=255) aTTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATcgc > 1:3595676/1‑69 (MQ=255) aTTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATcgc > 1:204451/1‑69 (MQ=255) ttGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATcgcg > 1:1844680/1‑69 (MQ=255) gTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCgcgc < 1:3232336/68‑1 (MQ=255) ttGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCt < 1:1599737/68‑1 (MQ=255) tGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTa < 1:1646071/69‑1 (MQ=255) tGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCtt < 1:106881/68‑1 (MQ=255) tGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTa > 1:1779597/1‑69 (MQ=255) tGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTa > 1:2708718/1‑69 (MQ=255) cGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATc < 1:578984/68‑1 (MQ=255) cGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATc < 1:3586264/68‑1 (MQ=255) cGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATc < 1:1744007/68‑1 (MQ=255) gCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATcc < 1:2316470/68‑1 (MQ=255) cTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCt < 1:2162479/56‑1 (MQ=255) gcAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAACGGATGAAGCGTCAg > 1:2005109/1‑69 (MQ=255) aGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGt > 1:2988329/1‑69 (MQ=255) aGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGt > 1:477925/1‑69 (MQ=255) aaGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGa > 1:2539495/1‑68 (MQ=255) cGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGATTCa < 1:2663574/69‑1 (MQ=255) | CCATTCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGATTCA > W3110S.gb/2307246‑2307375 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |