| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,313,823 | T→C | 62.2% | E349G (GAA→GGA) | yojL ← | predicted thiamine biosynthesis lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,313,823 | 0 | T | C | 62.2% | 23.0 / 30.9 | 32 | E349G (GAA→GGA) | yojL | predicted thiamine biosynthesis lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (6/6); new base C (8/11); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCT > W3110S.gb/2313755‑2313872 | tAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAAttttttt < 1:1026958/69‑1 (MQ=255)tAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAAttttttt < 1:1652297/69‑1 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTg > 1:3388837/1‑68 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGa < 1:88635/69‑1 (MQ=255) aGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGAc < 1:1157512/69‑1 (MQ=255) aGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGAc < 1:2031049/69‑1 (MQ=255) gCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGa < 1:34098/67‑1 (MQ=255) gCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGAc < 1:135180/68‑1 (MQ=255) tAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGaa > 1:1195032/1‑69 (MQ=255) cGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTaaa > 1:1690802/1‑69 (MQ=255) cGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTa < 1:2140165/67‑1 (MQ=255) cACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt > 1:1710816/1‑69 (MQ=255) aCCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:2725188/68‑1 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtggtg < 1:333032/69‑1 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtggt < 1:1526194/68‑1 (MQ=255) cACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGAc > 1:2120448/1‑69 (MQ=255) cACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGAc > 1:824916/1‑69 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtg > 1:3542648/1‑61 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtg > 1:54836/1‑61 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtg > 1:2257654/1‑61 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtg > 1:2790667/1‑61 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACtg < 1:1475493/61‑1 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACa > 1:378522/1‑69 (MQ=255) gCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:3535462/60‑1 (MQ=255) gCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATc < 1:3557303/69‑1 (MQ=255) cAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtggt > 1:911937/1‑62 (MQ=255) cAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtgg > 1:1807624/1‑61 (MQ=255) cAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGATGACAAGGAAGCTTTTAAACTgtggt > 1:2206673/1‑62 (MQ=255) tCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCt < 1:961880/69‑1 (MQ=255) tCTTGCGCTTTAATTTTTTACGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCt < 1:1304488/69‑1 (MQ=255) cTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTgtggt < 1:2045641/48‑1 (MQ=255) cTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTgtggt < 1:3658078/48‑1 (MQ=255) cTTGCGCTTTAATTTTTTCCGGTGACAATGAAGCTTTTAAACTGTGGTGACATCCAg < 1:3467983/57‑1 (MQ=255) ttGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATcc < 1:212496/54‑1 (MQ=255) | TAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCT > W3110S.gb/2313755‑2313872 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |