| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,315,568 | A→G | 51.9% | G172G (GGT→GGC) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,315,568 | 0 | A | G | 51.9% | 5.8 / 76.2 | 54 | G172G (GGT→GGC) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base A (16/10); new base G (13/15); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGT > W3110S.gb/2315506‑2315633 | gCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTgg > 1:725759/1‑69 (MQ=255)gCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTg > 1:3647165/1‑68 (MQ=255) cGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGt > 1:1781238/1‑69 (MQ=255) gACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGa < 1:2553123/68‑1 (MQ=255) tgttAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCaa > 1:2681700/1‑69 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc > 1:111336/1‑52 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc > 1:2827013/1‑52 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc > 1:1603227/1‑52 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc > 1:2098474/1‑52 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAcc > 1:223719/1‑51 (MQ=255) aCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTa > 1:1339635/1‑42 (MQ=255) aCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTa > 1:1476520/1‑42 (MQ=255) aCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTa > 1:1864297/1‑42 (MQ=255) aCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAgg > 1:352524/1‑69 (MQ=255) aCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAgg > 1:2952326/1‑69 (MQ=255) aCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAgg > 1:2017196/1‑69 (MQ=255) aCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAg > 1:1265703/1‑68 (MQ=255) tAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGtt < 1:3404276/58‑1 (MQ=255) tAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGtt < 1:17433/58‑1 (MQ=255) tAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGtt < 1:2041530/58‑1 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGtt < 1:944820/57‑1 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGtt < 1:3518990/57‑1 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCa > 1:635000/1‑67 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGa < 1:3157406/46‑1 (MQ=255) gTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAg < 1:2522206/54‑1 (MQ=255) gTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAg < 1:3012761/54‑1 (MQ=255) gTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCa > 1:2610553/1‑66 (MQ=255) gTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCa > 1:3639955/1‑66 (MQ=255) gTAAAGCCTTCACCAGATGGCTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAg < 1:1290888/54‑1 (MQ=38) aaaGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAg < 1:1664601/69‑1 (MQ=255) aaaGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAg < 1:3173259/69‑1 (MQ=255) aaaGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa < 1:2321199/68‑1 (MQ=255) aaaGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa < 1:3313079/68‑1 (MQ=255) aaaGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAg < 1:2286998/69‑1 (MQ=255) aaGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGa > 1:1303269/1‑69 (MQ=255) aaGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGa > 1:220483/1‑69 (MQ=255) aaGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAg < 1:2242265/68‑1 (MQ=255) aGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGtt < 1:2568933/52‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaaga < 1:1904114/67‑1 (MQ=255) cAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag < 1:1173264/68‑1 (MQ=255) aGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:3175877/69‑1 (MQ=255) gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCATACCGAAGAAGTCa > 1:1001767/1‑69 (MQ=255) gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa > 1:3166665/1‑69 (MQ=255) gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGACGTCAACCAGACCGAAGAAGtaa > 1:1891791/1‑67 (MQ=255) tGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt > 1:281930/1‑69 (MQ=255) tGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt > 1:3635668/1‑69 (MQ=255) tGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACAGAAGAAGTCAgt > 1:1302940/1‑69 (MQ=255) gggTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCTAAGAAGTc < 1:2647829/65‑1 (MQ=255) gggTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:1083620/65‑1 (MQ=255) tGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTAc < 1:1873142/69‑1 (MQ=255) cGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAg < 1:640165/46‑1 (MQ=255) cGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:3024853/68‑1 (MQ=255) tttGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg > 1:3530447/1‑64 (MQ=255) tttGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg > 1:2428799/1‑64 (MQ=255) tttGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg > 1:2223723/1‑64 (MQ=255) tttGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg > 1:1811559/1‑64 (MQ=255) tttGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt > 1:848241/1‑69 (MQ=255) | GCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGT > W3110S.gb/2315506‑2315633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |