| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,350,543 | G→A | 80.0% | V337I (GTA→ATA) | nrdA → | ribonucleoside diphosphate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,350,543 | 0 | G | A | 80.0% | 138.8 / 31.7 | 65 | V337I (GTA→ATA) | nrdA | ribonucleoside diphosphate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (5/8); new base A (23/29); total (28/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGC > W3110S.gb/2350485‑2350605 | ggTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATca > 1:1846289/1‑68 (MQ=255)ggTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATca > 1:3460862/1‑68 (MQ=255) gtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaacaa > 1:1490950/1‑69 (MQ=255) gtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATcaaca < 1:2047345/68‑1 (MQ=255) gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACt < 1:1948629/69‑1 (MQ=255) gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACt > 1:2197172/1‑69 (MQ=255) gAAAAACAACCGTGGTGTGGAAGGCAACAGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACt > 1:2614821/1‑69 (MQ=255) aaaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACt < 1:280856/68‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATcaacaa < 1:2412709/64‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATcaacaa < 1:2575583/64‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg < 1:53522/68‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg < 1:128753/68‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTA‑‑‑GGTACAAATCAACAAACTGATg < 1:1757499/68‑1 (MQ=255) aacaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:1336480/69‑1 (MQ=255) aacaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg > 1:1405542/1‑69 (MQ=255) aacaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg > 1:3702010/1‑69 (MQ=255) aacaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:2469406/69‑1 (MQ=255) aacaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:2195233/69‑1 (MQ=255) aacaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:1940831/69‑1 (MQ=255) acaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg > 1:1649181/1‑68 (MQ=255) acaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg > 1:3001049/1‑68 (MQ=255) acaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg > 1:46658/1‑68 (MQ=255) cGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATAc < 1:1186768/68‑1 (MQ=255) gtgtGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACt < 1:2783141/55‑1 (MQ=255) tgGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTc < 1:2813158/66‑1 (MQ=255) tgGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTc < 1:2644022/66‑1 (MQ=255) tgGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTc < 1:1584555/66‑1 (MQ=255) gAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTctgct > 1:3304083/1‑68 (MQ=255) gAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTctgct > 1:3502960/1‑68 (MQ=255) gAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTctgct > 1:2865319/1‑68 (MQ=255) aGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg > 1:2115185/1‑49 (MQ=255) aGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg > 1:3521505/1‑49 (MQ=255) aGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg > 1:1659907/1‑49 (MQ=255) aGGCAACCGCGTGCGTCATATGGACTAAGGGATACAAATCAACAAACTg > 1:1158942/1‑49 (MQ=255) cAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg > 1:3237329/1‑49 (MQ=255) cAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:1407149/49‑1 (MQ=255) cAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg > 1:1681584/1‑49 (MQ=255) cAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:2719341/49‑1 (MQ=255) cAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg > 1:2601416/1‑49 (MQ=255) cAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:2294950/49‑1 (MQ=255) aaCCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAgg > 1:3563393/1‑68 (MQ=255) cgcgTGCGTCATATGGACTACGGGGTACAAATCAACAACCTGATg < 1:1963/45‑1 (MQ=255) cgcgTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:3014654/45‑1 (MQ=255) cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAgg > 1:7444/1‑65 (MQ=255) cgtgcgtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa < 1:3037031/69‑1 (MQ=255) cgtgcgtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa < 1:2001491/69‑1 (MQ=255) gcgtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGatat < 1:2672417/69‑1 (MQ=255) cgtCATATGGACTACGGGATACAAATCAACAAACt > 1:605948/1‑35 (MQ=255) cgtCATATGGACTACGGGATACAAATCAACAAACt > 1:1302012/1‑35 (MQ=255) cgtCATATGGACTACGGGATACAAATCAACAAACt > 1:3085332/1‑35 (MQ=255) cgtCATATGGACTACGGGATACAAATCAACAAACt > 1:2791640/1‑35 (MQ=255) gtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATc < 1:2968647/68‑1 (MQ=255) tCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:3139183/37‑1 (MQ=255) atatGGACTACGGGATACAAATCAACAAACTGATg < 1:1589195/35‑1 (MQ=255) atatGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCAccc < 1:1785667/69‑1 (MQ=255) atGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTg > 1:3066306/1‑69 (MQ=255) tGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGaaa < 1:643750/50‑1 (MQ=255) tGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGaaa < 1:819722/50‑1 (MQ=255) gACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTc > 1:218628/1‑69 (MQ=255) gACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCAc < 1:1436659/62‑1 (MQ=255) gACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt < 1:3124213/68‑1 (MQ=255) cTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTaaa < 1:1418430/52‑1 (MQ=255) cTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa < 1:3223773/52‑1 (MQ=255) cTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa < 1:3465990/52‑1 (MQ=255) tACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGc < 1:655136/69‑1 (MQ=255) gTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGatat > 1:3252325/1‑50 (MQ=255) | GGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGC > W3110S.gb/2350485‑2350605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |