| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,397,191 | A→G | 80.0% | F174S (TTC→TCC) | nuoM ← | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,397,191 | 0 | A | G | 80.0% | 100.1 / 17.9 | 45 | F174S (TTC→TCC) | nuoM | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Reads supporting (aligned to +/- strand): ref base A (3/6); new base G (21/15); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAG > W3110S.gb/2397129‑2397255 | cAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTg < 1:2180530/68‑1 (MQ=255)cAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTg < 1:2076227/68‑1 (MQ=255) aaaaaCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTg < 1:1493729/67‑1 (MQ=255) aaaaCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGTGTGTAAATGAAGGACTTGGtt < 1:1316724/69‑1 (MQ=255) aaaaCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGtt < 1:656216/69‑1 (MQ=255) aaaaCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGtt < 1:263865/69‑1 (MQ=255) aaaaCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGtt < 1:1151422/69‑1 (MQ=255) cAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTgccgc < 1:2020872/69‑1 (MQ=255) cgcCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGt < 1:1036954/69‑1 (MQ=255) aaTCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtttt < 1:2212485/69‑1 (MQ=255) aTCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTa < 1:3006632/69‑1 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtttt > 1:444942/1‑66 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGttt > 1:3504842/1‑65 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:376512/1‑69 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:148004/1‑69 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTgccg < 1:374039/49‑1 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTgccg < 1:167426/49‑1 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTgccg < 1:1300409/49‑1 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATa > 1:1633587/1‑57 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATa > 1:1971838/1‑57 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTa > 1:600197/1‑66 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTa > 1:109545/1‑66 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:3095826/1‑68 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACATGGTTGCCGCCGTGATACGCGTTTTAcc > 1:2393661/1‑68 (MQ=255) cATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGccgccg > 1:2927847/1‑50 (MQ=255) cATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGt > 1:3533963/1‑51 (MQ=255) cATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGt > 1:3052179/1‑51 (MQ=255) cATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGt > 1:196906/1‑51 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:128760/68‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1453862/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1486186/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:2337171/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:531764/69‑1 (MQ=255) gCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt > 1:2817813/1‑68 (MQ=255) ccACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGttt < 1:1193441/52‑1 (MQ=255) cACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt > 1:210833/1‑69 (MQ=255) aCTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGttt > 1:1308507/1‑50 (MQ=255) cGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTa < 1:2701247/49‑1 (MQ=255) aaTGAAGGACTTGGTTGCCGCCGTGCTACGCGTTTTACCGTCa > 1:562235/1‑43 (MQ=38) aaTGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCttt > 1:3658118/1‑51 (MQ=255) aaTGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt > 1:1304449/1‑50 (MQ=255) aaTGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt > 1:1840797/1‑50 (MQ=255) aaTGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt > 1:1158463/1‑50 (MQ=255) aaTGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCg > 1:2558731/1‑67 (MQ=255) aaTGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCg > 1:2166051/1‑67 (MQ=255) gaaggaCTTGGTTGCCGCCGTGATACGCGTTTTACCGTCATAGGCTTTATGCCCCCACAGTGCGATCAg < 1:2663127/69‑1 (MQ=255) | CAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAG > W3110S.gb/2397129‑2397255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |