| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,427,890 | T→G | 100% | I246S (ATT→AGT) | yfcH → | conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,427,890 | 0 | T | G | 82.7% | 65.9 / 2.7 | 23 | I246S (ATT→AGT) | yfcH | conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base T (3/1); new base G (4/15); total (7/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCCGGTACGCAATGAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCATTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAGTACT > W3110S.gb/2427831‑2427944 | ccgggTACGCAATGAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCATTTTGCGCGt > 1:1593599/4‑69 (MQ=255)cccGGTACGCAATGAACAATTTGCCCATGCACTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGcgcg > 1:1968716/1‑68 (MQ=255) tGAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGc < 1:2094257/69‑1 (MQ=255) aaTTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCATTTTGCGCGTCCCCGCGACCGCCATTc > 1:2580196/1‑69 (MQ=255) aaTTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCAGCCATTTTGCGCGTCCCCGCGACCGCCATTc > 1:668393/1‑69 (MQ=255) cATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCGCGCGACCGCCATTCGACTGtt < 1:2925475/68‑1 (MQ=255) cATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt < 1:2674304/68‑1 (MQ=255) cATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt < 1:1199300/68‑1 (MQ=255) cATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTa < 1:195441/69‑1 (MQ=255) aTGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTaa < 1:629788/69‑1 (MQ=255) aTGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTaa < 1:253016/69‑1 (MQ=255) cgcTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt < 1:1412144/64‑1 (MQ=255) cgcTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt < 1:1377392/64‑1 (MQ=255) cgcTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATgg > 1:2047107/1‑69 (MQ=255) cATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATc > 1:1001002/1‑68 (MQ=255) cATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATc > 1:2560499/1‑68 (MQ=255) cATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACT‑TTAATGGGCGAATCtt < 1:1586715/69‑1 (MQ=255) aTGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCTTTCGACTGTTAATGGGCGAATCtt < 1:650643/69‑1 (MQ=255) aTGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCtt < 1:1792446/69‑1 (MQ=255) aTGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCtt < 1:3318522/69‑1 (MQ=255) aTGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCtt < 1:1609678/69‑1 (MQ=255) cgcTGCATCGCCCGGCCATTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAg < 1:567517/69‑1 (MQ=255) gCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAGTACt < 1:325732/69‑1 (MQ=255) | CCCGGTACGCAATGAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCATTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAGTACT > W3110S.gb/2427831‑2427944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |