| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,501,495 | G→A | 60.0% | S135S (TCG→TCA) | ddg → | palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,501,495 | 0 | G | A | 60.0% | 14.1 / 33.0 | 30 | S135S (TCG→TCA) | ddg | palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (7/5); new base A (6/12); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACAT > W3110S.gb/2501437‑2501558 | ttAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTgg > 1:1542177/1‑69 (MQ=255) acaAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGt < 1:3492000/69‑1 (MQ=255) aaaTGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGGCGCGTGa < 1:1425342/69‑1 (MQ=255) aaaTGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGa < 1:1614616/69‑1 (MQ=255) aaaTGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGa < 1:560271/69‑1 (MQ=255) attGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGt < 1:2164300/64‑1 (MQ=255) aaTGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGt < 1:3302316/66‑1 (MQ=255) tGCAAACTCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATgg < 1:2924707/69‑1 (MQ=255) tGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATgg < 1:3583270/69‑1 (MQ=255) aTGGTTGTCGGCGTCCATTTTATGTCACTGGAACTgg < 1:341902/37‑1 (MQ=255) aTGGTTGTCGGCGTCCATTTTATGTCACTGGAACTgg < 1:740137/37‑1 (MQ=255) aTGGTTGTCGGCGTCCATTTTATGTCACTGGAACTgg < 1:606727/37‑1 (MQ=255) aTGGTTGTCGGCGTCCATTTTATGTCACTGGAACTgg < 1:2894168/37‑1 (MQ=255) aTGGTTGTCGGCGTCCATTTTATGTCACTGGAACTgg < 1:1969703/37‑1 (MQ=255) aTGGTTGTCGGCGTCCATTTTATGTCACTGGAACTg > 1:1463559/1‑36 (MQ=255) aTGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCcgcg > 1:997416/1‑46 (MQ=255) ggTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAatga > 1:3271950/1‑68 (MQ=255) ggCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAat > 1:41453/1‑59 (MQ=255) ccATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATc < 1:848472/68‑1 (MQ=255) cATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGATACCTATCg > 1:397327/1‑68 (MQ=255) cATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGccaacca > 1:1497388/1‑51 (MQ=255) ttttATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTc < 1:484263/68‑1 (MQ=255) tttATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCca > 1:3427998/1‑69 (MQ=255) ttATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGcc > 1:358112/1‑42 (MQ=255) ttATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGcc > 1:1678137/1‑42 (MQ=255) ttATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCca > 1:383250/1‑68 (MQ=255) ttATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCca > 1:663862/1‑68 (MQ=255) ttATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGccaacca < 1:2601492/47‑1 (MQ=255) tATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATc > 1:1092642/1‑62 (MQ=255) tGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACat < 1:2235704/68‑1 (MQ=255) | TTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACAT > W3110S.gb/2501437‑2501558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |