| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,541,664 | G→A | 78.9% | E129K (GAA→AAA) | crr → | glucose‑specific enzyme IIA component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,541,664 | 0 | G | A | 78.9% | 105.5 / 25.7 | 52 | E129K (GAA→AAA) | crr | glucose‑specific enzyme IIA component of PTS |
| Reads supporting (aligned to +/- strand): ref base G (7/4); new base A (17/24); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAAC > W3110S.gb/2541604‑2541727 | gaagaaGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAaga < 1:508485/65‑1 (MQ=255)gaagaaGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAaga < 1:136354/65‑1 (MQ=255)gaagaaGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAaga < 1:2875505/65‑1 (MQ=255) gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGcc < 1:3121545/69‑1 (MQ=255) agGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:413320/69‑1 (MQ=255) agGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:3621395/69‑1 (MQ=255) ggTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGcc < 1:2878731/66‑1 (MQ=255) ggTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGACGCTGCTGAAAGAGAAAGcc < 1:2063668/66‑1 (MQ=255) tCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTc < 1:2842360/69‑1 (MQ=255) cAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTc < 1:1351806/68‑1 (MQ=255) cAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCt > 1:1772115/1‑69 (MQ=255) cAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCt > 1:2499146/1‑69 (MQ=255) cAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCt > 1:2711790/1‑69 (MQ=255) cAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCt > 1:1870065/1‑69 (MQ=255) cAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCt > 1:369735/1‑69 (MQ=255) aGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTcga > 1:1617764/1‑67 (MQ=255) aGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTcga > 1:1565460/1‑67 (MQ=255) aGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTc > 1:1038566/1‑67 (MQ=255) aGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTa < 1:3026480/69‑1 (MQ=255) aGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTa < 1:1043523/69‑1 (MQ=255) aGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTa < 1:3550252/69‑1 (MQ=255) aGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTa > 1:258470/1‑69 (MQ=255) cgTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:1645119/60‑1 (MQ=255) gAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGa > 1:957598/1‑68 (MQ=255) ggCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCt > 1:1524679/1‑59 (MQ=255) cGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:223448/48‑1 (MQ=255) gATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgt < 1:873017/68‑1 (MQ=255) aTACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGTTTGTTa < 1:4739/69‑1 (MQ=255) aTACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTa < 1:1884768/69‑1 (MQ=255) aTACTGTCATTGAATTTGATCTGCCGCTGCTGAAaga > 1:499427/1‑37 (MQ=255) aTACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt < 1:2728366/68‑1 (MQ=255) gTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGcc > 1:1725665/1‑39 (MQ=255) gTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGcc > 1:800541/1‑39 (MQ=255) gTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGcc > 1:1357322/1‑39 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt > 1:2297315/1‑60 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgt > 1:2389860/1‑59 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTa > 1:2110532/1‑61 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTa > 1:2899560/1‑61 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTa > 1:2420245/1‑61 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTa > 1:3280080/1‑61 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGAAAGATAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCaa < 1:3187676/68‑1 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa > 1:717480/1‑38 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt < 1:3605970/60‑1 (MQ=255) gAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttg < 1:270122/55‑1 (MQ=255) gAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttg < 1:899526/55‑1 (MQ=255) gAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATg < 1:1133075/69‑1 (MQ=255) tGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGa > 1:2607794/1‑69 (MQ=255) cTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTAtc < 1:1869598/51‑1 (MQ=255) cTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTAtc < 1:3173317/51‑1 (MQ=255) gctgctGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCa < 1:2015329/65‑1 (MQ=255) gctgctGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCa < 1:587157/65‑1 (MQ=255) gctgAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAAc > 1:3670031/1‑68 (MQ=255) | GAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAAC > W3110S.gb/2541604‑2541727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |