| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,589,664 | A→T | 57.1% | intergenic (+300/‑239) | acrD → / → yffB | aminoglycoside/multidrug efflux system/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,589,664 | 0 | A | T | 57.1% | 15.0 / 64.0 | 49 | intergenic (+300/‑239) | acrD/yffB | aminoglycoside/multidrug efflux system/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (7/14); new base T (10/18); total (17/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01 | |||||||||||
ATGACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTAAAATAC > W3110S.gb/2589599‑2589728 | aTGACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTa < 1:1143362/68‑1 (MQ=255)aTGACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTa < 1:1367977/68‑1 (MQ=255) gACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTacac > 1:2436101/1‑69 (MQ=255) aCTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGa < 1:3643094/63‑1 (MQ=255) ccTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa > 1:1174056/1‑69 (MQ=255) ggggACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTc < 1:951118/69‑1 (MQ=255) ggggACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTc < 1:2679047/69‑1 (MQ=255) ggggACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTc < 1:2362663/69‑1 (MQ=255) ggggACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTc < 1:1001004/69‑1 (MQ=255) tCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt > 1:275336/1‑68 (MQ=255) tCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt > 1:2719647/1‑68 (MQ=255) ttATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCAt > 1:30250/1‑69 (MQ=255) aTGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCa < 1:427390/66‑1 (MQ=255) gTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCAt < 1:1543474/65‑1 (MQ=255) gTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCAt < 1:716829/65‑1 (MQ=255) gTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCAt < 1:212051/65‑1 (MQ=255) gTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCAt < 1:1250541/65‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCAt < 1:3185100/64‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCAt < 1:2763507/64‑1 (MQ=255) aaTACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:1674256/68‑1 (MQ=255) tACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAAc < 1:2675626/44‑1 (MQ=255) tACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAAc < 1:2738532/44‑1 (MQ=255) tACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCt > 1:2785617/1‑69 (MQ=255) aTGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAAt < 1:2674145/68‑1 (MQ=255) aTGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCAAGATATTCATACGGCATCTAAt < 1:2368503/68‑1 (MQ=255) aTGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCAAGATATTCATACGGCATCTAAt < 1:2364410/68‑1 (MQ=255) gTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACt < 1:439032/68‑1 (MQ=255) gTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACt < 1:1045467/68‑1 (MQ=255) gTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACt < 1:2978835/68‑1 (MQ=255) gTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTg > 1:802342/1‑69 (MQ=255) gTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTg > 1:2928436/1‑69 (MQ=255) gTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGTATCTAATACTg > 1:1347878/1‑69 (MQ=255) ccTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGa < 1:1705650/68‑1 (MQ=255) ccTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGa < 1:3358342/68‑1 (MQ=255) gCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGAttt < 1:2540586/68‑1 (MQ=255) tgatgaTTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTc < 1:3192589/69‑1 (MQ=255) atgatTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTg > 1:997947/1‑69 (MQ=255) gatTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:3032667/39‑1 (MQ=255) gatTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:2998729/39‑1 (MQ=255) gatTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTc < 1:3343509/65‑1 (MQ=255) gatTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAACTc < 1:1674980/65‑1 (MQ=255) ttGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTaa > 1:1622687/1‑48 (MQ=255) ttGTATGTGATACACAGCAACATTTCGAGATATTCa > 1:2609622/1‑36 (MQ=255) ttGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTaa > 1:2708837/1‑48 (MQ=255) ttGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTaa > 1:2572005/1‑48 (MQ=255) ttGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTaa > 1:2059262/1‑48 (MQ=255) ttGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTaa < 1:74619/48‑1 (MQ=255) gTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTaaaa > 1:746965/1‑69 (MQ=255) gTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTaaaa > 1:1102939/1‑69 (MQ=255) tgtgTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTAAAATac < 1:3314534/69‑1 (MQ=255) | ATGACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTAAAATAC > W3110S.gb/2589599‑2589728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |