| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,594,104 | G→A | 53.3% | H138Y (CAT→TAT) | ypfI ← | predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,594,104 | 0 | G | A | 53.3% | ‑1.1 / 39.8 | 30 | H138Y (CAT→TAT) | ypfI | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (9/5); new base A (10/6); total (19/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCAG > W3110S.gb/2594041‑2594169 | agaATGGCTGGTTTTGCCGCCAGAGGATAGCCTCTTTATCCGCCGTAAGTACGCGTTTGAGATACTgg > 1:2107327/1‑68 (MQ=255) aTGGCTGGTTTTGCCTCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACaa < 1:416581/69‑1 (MQ=255) tGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACaaa > 1:2487370/1‑69 (MQ=255) gTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACaa > 1:3039902/1‑62 (MQ=255) tttGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATgcggcg > 1:1190901/1‑69 (MQ=255) cgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGc < 1:1702228/68‑1 (MQ=255) gccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGc < 1:326082/67‑1 (MQ=255) tAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAg > 1:199533/1‑69 (MQ=255) tAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAg > 1:258071/1‑69 (MQ=255) gCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATgcgg < 1:1170971/50‑1 (MQ=255) aTCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGc > 1:1484751/1‑48 (MQ=255) aTCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGc > 1:3697664/1‑48 (MQ=255) aTCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGc > 1:810866/1‑48 (MQ=255) aTCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGc > 1:1897080/1‑48 (MQ=255) aTCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCa > 1:2885344/1‑49 (MQ=255) aTCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCa > 1:1351331/1‑49 (MQ=255) aGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCaa > 1:568592/1‑40 (MQ=255) aGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCaa > 1:1797172/1‑40 (MQ=255) gTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAggg > 1:2093932/1‑38 (MQ=255) gTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAggg > 1:1287392/1‑38 (MQ=255) gTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAggg < 1:72526/38‑1 (MQ=255) gTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAggg < 1:2006526/38‑1 (MQ=255) gTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAggg > 1:2729260/1‑38 (MQ=255) gTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAggg < 1:2176736/38‑1 (MQ=255) gTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCgg < 1:467921/69‑1 (MQ=255) tttGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCgg < 1:3407502/68‑1 (MQ=255) tGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGc > 1:3328388/1‑67 (MQ=255) tGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCAt < 1:2949273/69‑1 (MQ=255) tGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCcagcgcagcg < 1:568409/60‑1 (MQ=255) gaTACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCAg > 1:341994/1‑69 (MQ=255) | AGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCAG > W3110S.gb/2594041‑2594169 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |