| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,599,304 | G→A | 100% | M57I (ATG→ATA) | bcp → | thiol peroxidase, thioredoxin‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,599,304 | 0 | G | A | 81.8% | 71.0 / 11.6 | 33 | M57I (ATG→ATA) | bcp | thiol peroxidase, thioredoxin‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/6); new base A (13/14); total (13/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.98e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TATTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTC > W3110S.gb/2599239‑2599367 | tATTTCTACCCGAAAGCCATGACCCCCGGCTGTACGGTACAGGCCTGCGGCTTACGCGATAACATAGAt < 1:3605286/69‑1 (MQ=255) tttCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATg < 1:1373817/68‑1 (MQ=255) tttCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATg < 1:1602099/68‑1 (MQ=255) tttCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATg < 1:3154653/68‑1 (MQ=255) tCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAg > 1:232196/1‑68 (MQ=255) gAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGaaaaa < 1:1960983/69‑1 (MQ=255) gAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGaaaaa < 1:1984604/69‑1 (MQ=255) gAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGaaaaa < 1:1999461/69‑1 (MQ=255) aCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGtt > 1:121031/1‑69 (MQ=255) aCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGtt < 1:579078/69‑1 (MQ=255) ccccGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCggg < 1:708170/63‑1 (MQ=255) tGTACCGTACAGGCCTGCGGCTTACGCGATAACATg < 1:3656038/36‑1 (MQ=255) ccGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGt < 1:3584104/68‑1 (MQ=255) ccGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGt > 1:483274/1‑68 (MQ=255) ccGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCAGGCGTTGATGTGCTGGGt > 1:2193474/1‑68 (MQ=255) cGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGTTg < 1:732430/56‑1 (MQ=255) tACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATc > 1:2463961/1‑68 (MQ=255) cAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCggg < 1:698592/47‑1 (MQ=255) cAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCggg < 1:1339997/47‑1 (MQ=255) cAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGtt < 1:54479/51‑1 (MQ=255) cAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGc > 1:3575980/1‑69 (MQ=255) cAGGCCT‑CGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCggg < 1:2890762/46‑1 (MQ=255) gCCTGCGGCTTACGCGATAACATAGATGAGTTGaaa > 1:879055/1‑36 (MQ=255) gCCTGCGGCTTACGCGATAACATAGATGAGTTGaaa > 1:894274/1‑36 (MQ=255) gCCTGCGGCTTACGCGATAACATAGATGAGTTGaaa > 1:3433315/1‑36 (MQ=255) gCCTGCGGCTTACGCGATAACATAGATGAGTTGaaa > 1:1946027/1‑36 (MQ=255) tGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGAt < 1:1747324/69‑1 (MQ=255) ttACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGtt < 1:2866010/39‑1 (MQ=255) cgcgATAACATGGATGAGTTGAACAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGa < 1:1487146/59‑1 (MQ=255) cgcgATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGa < 1:2711484/59‑1 (MQ=255) cgcgATAACATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGa > 1:2112459/1‑68 (MQ=255) cgATAACATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaa < 1:3124498/68‑1 (MQ=255) aaCATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAActc > 1:2454091/1‑69 (MQ=255) aCATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTAt > 1:23883/1‑43 (MQ=255) | TATTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTC > W3110S.gb/2599239‑2599367 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |