| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,609,568 | T→C | 17.5% | V69V (GTT→GTC) | hyfI → | hydrogenase 4, Fe‑S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,609,568 | 0 | T | C | 17.5% | 145.5 / 25.1 | 63 | V69V (GTT→GTC) | hyfI | hydrogenase 4, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base T (21/31); new base C (4/7); total (25/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
AAATCGAAATTTTTGCTGCCATTACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTG > W3110S.gb/2609504‑2609635 | aaaTCGAAATTTTTGCTGCCATTACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTCtcat < 1:2557622/69‑1 (MQ=255)aaaTCGAAATTTTTGCTGCCATTACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTCtcat < 1:2455890/69‑1 (MQ=255) aTCGAAATTTTTGCTGCCATTACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTTTCtcat < 1:476721/67‑1 (MQ=255) gAAATTTTTGCTGCCATTACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTtcatc > 1:885453/1‑65 (MQ=255) gAAATTTTTGCTGCCATTACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTtcatc > 1:3454325/1‑65 (MQ=255) gAAATTTTTGCTGCCATTACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTtcatc > 1:289200/1‑65 (MQ=255) ctgcCATTACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGcc < 1:1327972/68‑1 (MQ=255) tgcCATTACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGcc < 1:142531/67‑1 (MQ=255) ttACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATAttt < 1:1022977/69‑1 (MQ=255) ttACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATAttt < 1:1450623/69‑1 (MQ=255) ttACACCAGTATTCGACGCAGAACGTTTTGGCATTAAAGTTGTTTCATCACCGCGTCACGCCGATAttt < 1:3392070/69‑1 (MQ=255) acacCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGttt < 1:851677/43‑1 (MQ=255) acacCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATAtttt > 1:3221815/1‑68 (MQ=255) acacCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATAtttt > 1:2339983/1‑68 (MQ=255) acacCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTCtcat < 1:2995340/46‑1 (MQ=255) acacCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTCtcat < 1:2697548/46‑1 (MQ=255) aGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTa < 1:3468880/67‑1 (MQ=255) aTTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTAc > 1:784552/1‑69 (MQ=255) ttCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACt > 1:2645469/1‑69 (MQ=255) ttCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACt > 1:3604487/1‑69 (MQ=255) ttCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACt > 1:2082024/1‑69 (MQ=255) ttCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACt > 1:2162647/1‑69 (MQ=255) ttCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACt > 1:2225702/1‑69 (MQ=255) ttCGACGCAGAACGTTTTGGCATTAAGGTTGTCtcatc > 1:1392368/1‑38 (MQ=255) ttCGACGCAGAACGTTTTGGCATTAAGGTTGTCtcatc > 1:819569/1‑38 (MQ=255) ttCGACGCAGAACGTTTTGGCATTAAGGTTGTCtcat > 1:1905536/1‑37 (MQ=255) ttCGACGCAGAACGTTTAGGCATTAAGGTTGTCtcatc > 1:3217948/1‑38 (MQ=38) cGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTgg < 1:3615044/69‑1 (MQ=255) cGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTgg < 1:3285248/69‑1 (MQ=255) cGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTgg < 1:1137784/69‑1 (MQ=255) cGACGCAGAACGTTTTGGCATTAAGGTTGTCTCATCACCGCGTCACGCCGATATTTTGt < 1:361642/59‑1 (MQ=255) gACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTgg > 1:433859/1‑68 (MQ=255) cGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGgcgc < 1:67951/69‑1 (MQ=255) cGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGgcgc < 1:3517225/69‑1 (MQ=255) cGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGgcgc < 1:2586128/69‑1 (MQ=255) cGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGgcgc < 1:3096621/69‑1 (MQ=255) cGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGgcgc < 1:365426/69‑1 (MQ=255) gCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGgcgc < 1:719876/68‑1 (MQ=255) gCAGAACGTTTTGGCATTAAGGTTGTCTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCa < 1:1008378/69‑1 (MQ=255) aGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGt < 1:1304423/69‑1 (MQ=255) aCGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTa < 1:1428552/52‑1 (MQ=255) aCGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCAc < 1:3260357/69‑1 (MQ=255) aCGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCAc > 1:3258331/1‑69 (MQ=255) gTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCa < 1:1131858/66‑1 (MQ=255) gTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCa < 1:89926/66‑1 (MQ=255) ggCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGc < 1:1277820/68‑1 (MQ=255) ggCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGc < 1:1629957/68‑1 (MQ=255) ggCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGc < 1:2324891/68‑1 (MQ=255) ggCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGc < 1:1745151/68‑1 (MQ=255) aTTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATg > 1:1686581/1‑69 (MQ=255) tAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCg < 1:3470180/69‑1 (MQ=255) tAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCg < 1:3716177/69‑1 (MQ=255) aaGGTTGTTTCATCACCGCGTCACGCCGATATTTTGtt < 1:13260/38‑1 (MQ=255) aaGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCa > 1:3405955/1‑51 (MQ=255) aaGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCa < 1:281001/51‑1 (MQ=255) aaGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGc > 1:331882/1‑62 (MQ=255) aaGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGc > 1:1113470/1‑62 (MQ=255) aaGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCg > 1:1669459/1‑68 (MQ=255) aaGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCg < 1:1142441/68‑1 (MQ=255) aaGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGt > 1:1300519/1‑69 (MQ=255) aaGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGt > 1:818596/1‑69 (MQ=255) aaGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTAATTACTGGCGCAGTCACCCGGGCGATGCGt > 1:2258063/1‑69 (MQ=255) ggTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTa < 1:2886980/68‑1 (MQ=255) ggTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTa < 1:3671398/68‑1 (MQ=255) gttTCATCACCGCGTCACGCCGATATTTTGTTAttt > 1:174435/1‑36 (MQ=255) ttCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTg < 1:981471/68‑1 (MQ=255) | AAATCGAAATTTTTGCTGCCATTACACCAGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTG > W3110S.gb/2609504‑2609635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |