| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,636,526 | C→T | 75.9% | L261L (CTG→CTA) | yfgL ← | protein assembly complex, lipoprotein component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,636,526 | 0 | C | T | 75.9% | 96.5 / 32.6 | 54 | L261L (CTG→CTA) | yfgL | protein assembly complex, lipoprotein component |
| Reads supporting (aligned to +/- strand): ref base C (5/8); new base T (21/20); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TCGCGTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATT > W3110S.gb/2636464‑2636589 | tCGCGTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAgcgc > 1:785990/1‑68 (MQ=255)tCGCGTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAgcgc > 1:2099610/1‑68 (MQ=255) cgTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGaa < 1:2985147/68‑1 (MQ=255) cgTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGaa < 1:2251514/68‑1 (MQ=255) cgTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGaa < 1:3150132/68‑1 (MQ=255) tCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaaca < 1:1460233/68‑1 (MQ=255) tCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaaca < 1:3226133/68‑1 (MQ=255) tCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAaacaa < 1:2155016/69‑1 (MQ=255) cacaTAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAaacaa < 1:377774/67‑1 (MQ=255) aaTCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGtt > 1:2685530/1‑69 (MQ=255) aaTCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGtt > 1:561083/1‑69 (MQ=255) cACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGcgcg < 1:2923417/47‑1 (MQ=255) cACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTaacaac < 1:3711952/67‑1 (MQ=255) gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaacaa < 1:856961/50‑1 (MQ=255) gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaacaa < 1:1531195/50‑1 (MQ=255) gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaacaa < 1:423209/50‑1 (MQ=255) gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGtt > 1:2859702/1‑57 (MQ=255) gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGtt < 1:2385758/57‑1 (MQ=255) gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGtt < 1:2457207/57‑1 (MQ=255) gcgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACggg < 1:759939/69‑1 (MQ=255) cgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACggg < 1:3200872/68‑1 (MQ=255) gATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaacaa < 1:3594655/45‑1 (MQ=255) aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGt < 1:964384/66‑1 (MQ=255) cAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg > 1:1890257/1‑68 (MQ=255) cAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAacgactg < 1:3429656/60‑3 (MQ=255) cAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACgg < 1:1063568/60‑1 (MQ=255) cAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACgg > 1:2032882/1‑60 (MQ=255) cAAGCGCCGACAGGTTACCATTATAGGCCAGCGCGAAaacaa > 1:48950/1‑42 (MQ=255) gcgcCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTaac < 1:1515458/49‑1 (MQ=255) aGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGTGAGTCGTGTCa > 1:1060863/1‑60 (MQ=255) aGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgt > 1:2237050/1‑56 (MQ=255) aGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg > 1:781102/1‑57 (MQ=255) aGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCg > 1:2974145/1‑66 (MQ=255) gTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCa < 1:2942286/68‑1 (MQ=255) gTTACCATTATAGGCTAGCGCGAAAACAACGACGTTAACAACGACGGGAGTCGTGTCAACATCGCTCa < 1:3475037/68‑1 (MQ=255) ttACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGa > 1:3070142/1‑69 (MQ=255) tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGTGAGTCGTGTCAACATCGCTCAGa < 1:3305410/68‑1 (MQ=255) tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCa > 1:1451872/1‑56 (MQ=255) tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGTTCAGAc > 1:190564/1‑69 (MQ=255) tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGa < 1:1138657/68‑1 (MQ=255) tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGa < 1:2163647/68‑1 (MQ=255) aCCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg < 1:1026466/69‑1 (MQ=255) tataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGTGAGTCGTGTCAACAt > 1:3178845/1‑54 (MQ=255) tataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCaa > 1:752665/1‑51 (MQ=255) tataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCaa > 1:1894369/1‑51 (MQ=255) tataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCa > 1:2685809/1‑50 (MQ=255) tataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACa > 1:1474783/1‑53 (MQ=255) tataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACAt > 1:1730869/1‑54 (MQ=255) tataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACAt > 1:1243677/1‑54 (MQ=255) tataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACAt > 1:722802/1‑54 (MQ=255) ataGGCTAGCGCGAAAACAACGCCGTTAACAACGCCGGGAGTCGTGTc > 1:2766038/1‑48 (MQ=255) ataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc < 1:3016467/48‑1 (MQ=255) ataGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCg > 1:2529922/1‑44 (MQ=255) taGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAAtt > 1:2192894/1‑69 (MQ=255) | TCGCGTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATT > W3110S.gb/2636464‑2636589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |