| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,646,699 | C→G | 55.6% | Q1415H (CAG→CAC) | yfhM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,646,699 | 0 | C | G | 55.6% | 5.4 / 30.0 | 27 | Q1415H (CAG→CAC) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (6/6); new base G (5/10); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
TCGTACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGC > W3110S.gb/2646632‑2646759 | tCGTACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCctgc > 1:1284070/1‑68 (MQ=255) cGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGaa < 1:2084658/69‑1 (MQ=255) cAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAgtg > 1:3254142/1‑69 (MQ=255) aacaacGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGc < 1:2720268/69‑1 (MQ=255) acaacGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAg > 1:3414887/1‑67 (MQ=255) aacGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCaaa < 1:2012736/69‑1 (MQ=255) acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAg < 1:1900424/69‑1 (MQ=255) acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAg < 1:1706625/69‑1 (MQ=255) acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAg < 1:2889308/69‑1 (MQ=255) gcgTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTTGTGCGAAAGTGTGTTCAGCAAAgtg < 1:1097202/69‑1 (MQ=255) gcgTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:174147/69‑1 (MQ=255) gcgTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:3433108/69‑1 (MQ=255) gcgTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:3187646/69‑1 (MQ=255) gcgTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:243108/69‑1 (MQ=255) gcgTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAgtg < 1:533569/69‑1 (MQ=255) aCTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTg < 1:2218562/69‑1 (MQ=255) tCCTGCGTCGATAGCCAGCGTTCACCAACCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCa > 1:820740/1‑69 (MQ=255) tCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCa > 1:2166048/1‑69 (MQ=255) tCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGt > 1:2023095/1‑68 (MQ=255) tCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGt > 1:1939334/1‑68 (MQ=255) ttCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCa < 1:3078555/49‑1 (MQ=255) ttCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCa < 1:749824/49‑1 (MQ=255) ttCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTttc < 1:1315082/69‑1 (MQ=255) cACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTAttt > 1:1990028/1‑65 (MQ=255) cAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTcc > 1:2413756/1‑68 (MQ=255) cAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTcc > 1:3224840/1‑68 (MQ=255) cAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTcc > 1:2075437/1‑68 (MQ=255) gCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc > 1:426332/1‑66 (MQ=255) | TCGTACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGC > W3110S.gb/2646632‑2646759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |