| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,667,558 | T→C | 86.9% | intergenic (‑6/‑130) | hcaR ← / → hcaE | DNA‑binding transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,667,558 | 0 | T | C | 86.9% | 103.0 / 7.4 | 38 | intergenic (‑6/‑130) | hcaR/hcaE | DNA‑binding transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base C (20/13); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACC > W3110S.gb/2667496‑2667620 | ggTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCtt < 1:3019984/69‑1 (MQ=255) gTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTg < 1:1469556/69‑1 (MQ=255) gTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTg < 1:3420476/69‑1 (MQ=255) tAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCtt > 1:915762/1‑67 (MQ=255) aaGTTCAGTTCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCtttta > 1:2938612/1‑65 (MQ=255) aaGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTa > 1:310127/1‑69 (MQ=255) ccTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGaaaaaa > 1:925298/1‑68 (MQ=255) aCTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGc > 1:1867626/1‑69 (MQ=255) tGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCtt < 1:3401581/45‑1 (MQ=255) gCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCt > 1:1221615/1‑67 (MQ=255) gCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCt > 1:2123116/1‑67 (MQ=255) cgaAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGa < 1:444194/69‑1 (MQ=255) gaAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCa > 1:992688/1‑59 (MQ=255) gaAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCa > 1:841707/1‑59 (MQ=255) gaAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCa < 1:3013387/59‑1 (MQ=255) gaAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCa > 1:2232981/1‑59 (MQ=255) aTAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAAtt < 1:2936652/69‑1 (MQ=255) aTAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGt < 1:3594791/52‑1 (MQ=255) aTAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGt < 1:1607422/52‑1 (MQ=255) aTAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGaaa > 1:80310/1‑67 (MQ=255) aTAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAAtt > 1:2774470/1‑69 (MQ=255) aTAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAAtt > 1:721711/1‑69 (MQ=255) aTAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAAt > 1:1406148/1‑68 (MQ=255) aGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAAc < 1:1162466/48‑1 (MQ=255) aGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTa > 1:3248523/1‑68 (MQ=255) aGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTa < 1:58526/68‑1 (MQ=255) aGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTa > 1:349444/1‑68 (MQ=255) gTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGaaaa > 1:2906015/1‑42 (MQ=255) gTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGaaaa > 1:1097484/1‑42 (MQ=255) gTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGaaaa > 1:1554024/1‑42 (MQ=255) gTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGaaaa > 1:3322016/1‑42 (MQ=255) gTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGaaaa > 1:212395/1‑42 (MQ=255) aaTGCCGTAGTTCCATCACCTTCCCCTTGTTATCGaaaa < 1:1849958/39‑1 (MQ=255) tGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTca < 1:2999736/69‑1 (MQ=255) ccGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTcaca < 1:1764866/69‑1 (MQ=255) ccGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTcaca < 1:3638558/69‑1 (MQ=255) cATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAAtt > 1:324605/1‑48 (MQ=255) tCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaacc < 1:3174970/69‑1 (MQ=255) | GGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACC > W3110S.gb/2667496‑2667620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |