| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,669,838 | T→C | 66.1% | V92A (GTT→GCT) | hcaC → | 3‑phenylpropionate dioxygenase, predicted ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,669,838 | 0 | T | C | 66.1% | 68.9 / 53.4 | 59 | V92A (GTT→GCT) | hcaC | 3‑phenylpropionate dioxygenase, predicted ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base T (5/15); new base C (16/23); total (21/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
CCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAG > W3110S.gb/2669773‑2669901 | ccTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTg < 1:691922/68‑1 (MQ=255) cTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTg < 1:3138202/67‑1 (MQ=255) gAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAgg < 1:1152721/69‑1 (MQ=255) aaCGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAggt < 1:2247145/67‑1 (MQ=255) aaCGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAggt < 1:2526390/67‑1 (MQ=255) aaCGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAggt < 1:3258830/67‑1 (MQ=255) aaaGCGTTTTGCCTGCCCGCCTCCGATCCGCTCAGCAGTTATCCAGTACACGTTGAAGGTGGTGACAtt < 1:1171884/69‑1 (MQ=255) aaaGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACAtt < 1:1331410/69‑1 (MQ=255) aGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACAtttt > 1:1062101/1‑69 (MQ=255) aGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACAtttt > 1:2949640/1‑69 (MQ=255) aGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACAtttt > 1:1030325/1‑69 (MQ=255) cGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCa > 1:2904703/1‑69 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATc < 1:340052/67‑1 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATc < 1:1997809/67‑1 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGa > 1:1752736/1‑69 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGa > 1:1998467/1‑69 (MQ=255) gcctgccCGCCACCGATCCGGTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt > 1:2150505/1‑69 (MQ=255) gcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt > 1:1107436/1‑69 (MQ=255) cctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa > 1:3302062/1‑46 (MQ=255) cctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACtt < 1:142301/69‑1 (MQ=255) aCCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAgg > 1:2259457/1‑67 (MQ=255) gATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCg < 1:1749106/52‑1 (MQ=255) gATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCg > 1:510022/1‑52 (MQ=255) gATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCa > 1:2259543/1‑68 (MQ=255) gATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCa > 1:595796/1‑68 (MQ=255) aTCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGc < 1:1775150/69‑1 (MQ=255) aTCCGATCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGc < 1:148281/69‑1 (MQ=255) cGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCAt < 1:2446632/69‑1 (MQ=255) cGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCAt < 1:3261648/69‑1 (MQ=255) cGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCAt < 1:3609856/69‑1 (MQ=255) cGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCAt < 1:2271338/69‑1 (MQ=255) cGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCAt < 1:622768/69‑1 (MQ=255) gCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCAt < 1:3566055/68‑1 (MQ=255) ccacTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACtt < 1:1889157/46‑1 (MQ=255) ccacTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACtt < 1:642321/46‑1 (MQ=255) ccacTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACtt < 1:646887/46‑1 (MQ=255) ccacTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACtt < 1:3148029/46‑1 (MQ=255) ccacTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACtt < 1:731167/46‑1 (MQ=255) cacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg < 1:2402980/67‑1 (MQ=255) cacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg > 1:2469610/1‑67 (MQ=255) cAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg < 1:512228/59‑1 (MQ=255) cAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg < 1:2552768/59‑1 (MQ=255) cAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg < 1:3081423/59‑1 (MQ=255) cAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg < 1:1009824/59‑1 (MQ=255) cAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg < 1:2574186/59‑1 (MQ=255) tACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGc < 1:295519/48‑1 (MQ=255) tACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGc < 1:2819338/48‑1 (MQ=255) acacGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATg < 1:3311660/51‑1 (MQ=255) acacGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATg < 1:748934/51‑1 (MQ=255) acacGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATg < 1:1366741/51‑1 (MQ=255) acacGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATg < 1:1963927/51‑1 (MQ=255) acacGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATg < 1:421492/51‑1 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg > 1:2584388/1‑54 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg > 1:2428464/1‑54 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg > 1:1732750/1‑54 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg > 1:2901701/1‑54 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg > 1:3404162/1‑54 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAg > 1:1766612/1‑68 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAg > 1:913590/1‑68 (MQ=255) | CCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAG > W3110S.gb/2669773‑2669901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |