| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,697,845 | G→A | 36.1% | G144D (GGT→GAT) | yfhH → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,697,845 | 0 | G | A | 36.1% | 33.1 / 33.7 | 36 | G144D (GGT→GAT) | yfhH | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (16/7); new base A (8/5); total (24/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
AATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCA > W3110S.gb/2697785‑2697903 | aaTGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCtggtgg > 1:2329567/1‑69 (MQ=255)aaTGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCtggtgg > 1:1938138/1‑69 (MQ=255)aaTGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCtggtg > 1:503956/1‑68 (MQ=255) cGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGgcgc > 1:1254000/1‑68 (MQ=255) gTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTc < 1:3525971/58‑1 (MQ=255) aCAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtg < 1:619890/57‑1 (MQ=255) aCAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAAc > 1:123359/1‑69 (MQ=255) aCAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAAc < 1:3609012/69‑1 (MQ=255) aaTGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACtt > 1:2347666/1‑69 (MQ=255) aTGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACttt > 1:63290/1‑69 (MQ=255) aTGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACttt > 1:2075709/1‑69 (MQ=255) ttGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGcc > 1:2472837/1‑69 (MQ=255) ttGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGcc > 1:837031/1‑69 (MQ=255) gcgcTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTg < 1:759192/69‑1 (MQ=255) ctctGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGaa < 1:3696172/69‑1 (MQ=255) ctctGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGaa < 1:1252754/69‑1 (MQ=255) tctGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCggg > 1:949389/1‑41 (MQ=255) tctGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCggg > 1:1606981/1‑41 (MQ=255) cgcgGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCt < 1:1518498/67‑1 (MQ=255) cgcgGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCt < 1:946206/67‑1 (MQ=255) ggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtga > 1:1510710/1‑66 (MQ=255) ggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtga > 1:2366865/1‑66 (MQ=255) cggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAg < 1:2101458/69‑1 (MQ=255) ttCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCt < 1:3695867/55‑1 (MQ=255) cTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCtt > 1:2175268/1‑68 (MQ=255) cTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTc > 1:2614029/1‑69 (MQ=255) cTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTc > 1:3103997/1‑69 (MQ=255) cTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTc > 1:1198984/1‑69 (MQ=255) cTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTc > 1:3382353/1‑69 (MQ=255) cTGACCGGTATTGGCGCGTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTc > 1:2004818/1‑69 (MQ=255) gTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAAtgctg > 1:815058/1‑69 (MQ=255) gTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAAtgctg > 1:436458/1‑69 (MQ=255) gTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAAtgctg > 1:2239363/1‑69 (MQ=255) gTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAAtgctg > 1:2458336/1‑69 (MQ=255) ttGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg < 1:1024594/69‑1 (MQ=255) ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTc > 1:479527/1‑59 (MQ=255) ggCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:431085/68‑1 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCa > 1:2403330/1‑69 (MQ=255) | AATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCA > W3110S.gb/2697785‑2697903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |