Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,706,728 G→A 48.9% P228L (CCA→CTA)  rseB ← anti‑sigma factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,706,7280GA48.9% ‑0.7 / 64.5 45P228L (CCA→CTA) rseBanti‑sigma factor
Reads supporting (aligned to +/- strand):  ref base G (4/19);  new base A (13/9);  total (17/28)
Fisher's exact test for biased strand distribution p-value = 5.80e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

CGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAGGAACAGAAAGC  >  W3110S.gb/2706663‑2706784
                                                                 |                                                        
cGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCa                                                       <  1:1202865/69‑1 (MQ=255)
    aGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGCAACCa                                                   >  1:70243/1‑69 (MQ=255)
     ggCATTTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGCAACCAg                                                  <  1:3636747/69‑1 (MQ=255)
     ggCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGCAACCAg                                                  <  1:2620119/69‑1 (MQ=255)
       cATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAgg                                                 <  1:2255490/68‑1 (MQ=255)
        aTGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGCAACCAGGtt                                               <  1:2019673/69‑1 (MQ=255)
           ttgtCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGc                                            >  1:1204805/1‑69 (MQ=255)
           ttgtCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGCAACCAGGTTGGc                                            <  1:165481/69‑1 (MQ=255)
           ttgtCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGCAACCAGGTTGGc                                            <  1:1658133/69‑1 (MQ=255)
               ccATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTc                                         <  1:965658/68‑1 (MQ=255)
               ccATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTc                                         <  1:1897455/68‑1 (MQ=255)
               ccATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTc                                         <  1:1718532/68‑1 (MQ=255)
               ccATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTc                                         <  1:1488745/68‑1 (MQ=255)
                cATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTc                                         <  1:3005080/67‑1 (MQ=255)
                 aTCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGCAACCAg                                                  >  1:1792485/1‑57 (MQ=255)
                 aTCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGCAACCAg                                                  >  1:1225948/1‑57 (MQ=255)
                  tcgtcgGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGCAACCAGGTTGGCGTCCAg                                      >  1:423040/1‑68 (MQ=255)
                    gtcgGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAg                                                  <  1:517310/54‑1 (MQ=255)
                    gtcgGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAg                                                  <  1:2222726/54‑1 (MQ=255)
                    gtcgGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGc                                                        >  1:1069930/1‑48 (MQ=255)
                    gtcgGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGc                                                        >  1:2778129/1‑48 (MQ=255)
                    gtcgGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGc                                                        >  1:1340359/1‑48 (MQ=255)
                    gtcgGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGCAACCAGGTTGGCGTCCAGCTg                                   >  1:3348126/1‑69 (MQ=255)
                      cgGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGaa                                 <  1:530927/69‑1 (MQ=255)
                                cGTCGACTACTGGAAACTTCGCTAAAACCCTGTAGCAAcc                                                    >  1:2671420/1‑40 (MQ=255)
                                    gACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCt                          <  1:2326454/62‑1 (MQ=255)
                                     actactGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCt                          <  1:2099933/61‑1 (MQ=255)
                                        actGGAAACTTCGCTAAAACCCTGTAGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTAc               <  1:2684945/69‑1 (MQ=255)
                                        actGGAAACTTCGCTAAAACCCTGTAGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTAc               <  1:416148/69‑1 (MQ=255)
                                            gAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAgg            >  1:2978298/1‑68 (MQ=255)
                                            gAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAgg            >  1:2141995/1‑68 (MQ=255)
                                            gAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAgg            >  1:145793/1‑68 (MQ=255)
                                              aaCTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAAtt                               <  1:3722766/47‑1 (MQ=255)
                                               aCTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAGGAACa        <  1:540544/69‑1 (MQ=255)
                                                 ttCGCTAAAACCCTGTAGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCt                 >  1:1609064/1‑58 (MQ=255)
                                                 ttCGCTAAAACCCTGTAGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAGGAACa        >  1:1255799/1‑67 (MQ=255)
                                                 ttCGCTAAAACCCTGTAGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAGGAACAGa      <  1:1427705/69‑1 (MQ=255)
                                                    gCTAAAACCCTGTAGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAGGAACAGaaa    >  1:3612667/1‑68 (MQ=255)
                                                    gCTAAAACCCTGTAGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAGGAACAGaaa    >  1:3176357/1‑68 (MQ=255)
                                                      tAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAGGAACAGAAAGc  <  1:2202884/68‑1 (MQ=255)
                                                      tAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAGGAACAGAAAGc  <  1:227146/68‑1 (MQ=255)
                                                      tAAAACCCTGTGGAAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAGGAACAGAAAGc  <  1:1871351/68‑1 (MQ=255)
                                                      tAAAACCCTGTAGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAGGAACAGAAAGc  <  1:830924/68‑1 (MQ=255)
                                                          aCCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCt                          <  1:699727/40‑1 (MQ=255)
                                                          aCCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCt                          <  1:765569/40‑1 (MQ=255)
                                                                 |                                                        
CGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAATTTAGCTTTTTCACCTACAGGAACAGAAAGC  >  W3110S.gb/2706663‑2706784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: