| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,710,918 | A→T | 11.3% | V168E (GTG→GAG) | yfiC ← | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,710,918 | 0 | A | T | 11.3% | 144.7 / 10.2 | 57 | V168E (GTG→GAG) | yfiC | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (22/28); new base T (4/2); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGAT > W3110S.gb/2710851‑2710974 | cAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCAc < 1:2686299/69‑1 (MQ=255)cAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCAc < 1:1440635/69‑1 (MQ=255)cAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCAc < 1:799450/69‑1 (MQ=255)cAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCAc < 1:1482297/69‑1 (MQ=255)cAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCAc < 1:1659408/69‑1 (MQ=255) tAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGc > 1:2083363/1‑68 (MQ=255) tGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGaaa > 1:1855775/1‑69 (MQ=255) tGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGaaa > 1:1807105/1‑69 (MQ=255) tGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCtt > 1:3676744/1‑69 (MQ=255) tGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCtt > 1:2503947/1‑69 (MQ=255) tGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCtt > 1:2956527/1‑69 (MQ=255) tGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCtt > 1:457603/1‑69 (MQ=255) tGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCtt > 1:1223684/1‑69 (MQ=255) cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGTAGCACTACGCAGAAAAATCCct > 1:2789230/1‑64 (MQ=255) cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTa > 1:431770/1‑49 (MQ=255) cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGaaa < 1:496613/57‑1 (MQ=255) cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGaaa < 1:2534696/57‑1 (MQ=255) cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCctc < 1:3475901/65‑1 (MQ=255) cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCctc < 1:195662/65‑1 (MQ=255) cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCctc < 1:2358701/65‑1 (MQ=255) cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCctc < 1:2469760/65‑1 (MQ=255) cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTc < 1:1486978/68‑1 (MQ=255) cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTc > 1:2185724/1‑68 (MQ=255) cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTc > 1:3578750/1‑68 (MQ=255) tAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGt < 1:3214179/69‑1 (MQ=255) tAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGt < 1:1171125/69‑1 (MQ=255) tAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGt < 1:795749/69‑1 (MQ=255) tAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGt < 1:1471544/69‑1 (MQ=255) tGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTaa > 1:557559/1‑68 (MQ=255) ccAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGaaaa > 1:437251/1‑51 (MQ=255) ccAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATg > 1:2199360/1‑68 (MQ=255) ccAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATg > 1:3190279/1‑68 (MQ=255) ccAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATg > 1:2097993/1‑68 (MQ=255) tAAAACCATTACCAATCTGCTCTGGCAGGACTACGCAGAAAAATCCCTCTTCGGTAATGCACTccgccg < 1:743351/69‑1 (MQ=255) aaaCCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATGCACTccgc < 1:2952759/65‑1 (MQ=255) aaaCCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATGCACTccgc < 1:3617603/65‑1 (MQ=255) aaaCCATTACCAATCTGCTCTGGCAGCCCTACGCAGAAAAATCCCTCTTCGGTAATGCACTccgc < 1:885363/65‑1 (MQ=255) aaaCCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTccgccg > 1:3476887/1‑67 (MQ=255) aaaCCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTACGGTAATGCACTccgccg > 1:940910/1‑67 (MQ=255) aCCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCg > 1:2768520/1‑49 (MQ=255) aCCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTa > 1:87952/1‑52 (MQ=255) aCCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCa > 1:291378/1‑68 (MQ=255) aCCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCa > 1:2460764/1‑68 (MQ=255) cAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGc < 1:1031257/68‑1 (MQ=255) aTCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAAc < 1:3714839/69‑1 (MQ=255) aTCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAAc < 1:295816/69‑1 (MQ=255) aTCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAAc < 1:1727274/69‑1 (MQ=255) aTCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAAc < 1:1254557/69‑1 (MQ=255) tggtctGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGAt > 1:3253591/4‑69 (MQ=255) tGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAgaac < 1:3403521/65‑4 (MQ=255) tGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCCAc < 1:1593860/66‑1 (MQ=255) tGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAAc < 1:3620790/66‑1 (MQ=255) tGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAAc < 1:348532/66‑1 (MQ=255) tGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAAc < 1:759512/66‑1 (MQ=255) tGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAAc < 1:970315/66‑1 (MQ=255) tGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGAt > 1:2137217/1‑69 (MQ=255) tGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGAt > 1:2132114/1‑69 (MQ=255) | CAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGAT > W3110S.gb/2710851‑2710974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |