| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,717,229 | G→A | 59.4% | intergenic (‑45/‑162) | yfiF ← / → trxC | predicted methyltransferase/thioredoxin 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,717,229 | 0 | G | A | 59.4% | 9.9 / 29.8 | 28 | intergenic (‑45/‑162) | yfiF/trxC | predicted methyltransferase/thioredoxin 2 |
| Reads supporting (aligned to +/- strand): ref base G (2/9); new base A (8/8); total (10/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01 | |||||||||||
ACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAG > W3110S.gb/2717164‑2717293 | aCCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATcc > 1:3115712/1‑69 (MQ=255) ttcatttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCcgcgc > 1:805838/1‑69 (MQ=255) ttcatttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCcgcgc > 1:2001263/1‑69 (MQ=255) tcatttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCcgcgcg > 1:3273285/1‑69 (MQ=255) tcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTc > 1:2161272/1‑68 (MQ=255) aGCGCTGCGCGAAGCGCGCAGATTACCCGAACTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc < 1:3560540/69‑1 (MQ=255) aGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc > 1:1322107/1‑69 (MQ=255) aGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc > 1:1486580/1‑69 (MQ=255) aGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc > 1:3435210/1‑69 (MQ=255) gcgcTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc < 1:3085322/68‑1 (MQ=255) gcgcTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCg < 1:344990/69‑1 (MQ=255) tGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTa < 1:1325546/69‑1 (MQ=255) cgAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa < 1:281328/68‑1 (MQ=255) cgAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa < 1:2070070/68‑1 (MQ=255) cgAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt < 1:420205/69‑1 (MQ=255) aGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTAt > 1:3242001/1‑63 (MQ=255) cAGATTACCCGCAATCCGCGCTGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGa < 1:1863736/69‑1 (MQ=255) cAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa < 1:227713/59‑1 (MQ=255) cAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa < 1:438217/59‑1 (MQ=255) ttACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaa < 1:2720711/69‑1 (MQ=255) tACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTAt < 1:3087187/52‑1 (MQ=255) tACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTAt < 1:3091322/52‑1 (MQ=255) tACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTAt < 1:165525/52‑1 (MQ=255) tACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTAt < 1:1346231/52‑1 (MQ=255) tACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt < 1:637835/55‑1 (MQ=255) tACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaa > 1:2069714/1‑68 (MQ=255) cGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAg < 1:2558788/69‑1 (MQ=255) cGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAg < 1:1964375/69‑1 (MQ=255) | ACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAG > W3110S.gb/2717164‑2717293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |