| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 85,966 | T→C | 53.8% | F113L (TTT→CTT) | ilvI → | acetolactate synthase III, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 85,966 | 0 | T | C | 53.8% | 2.5 / 32.5 | 26 | F113L (TTT→CTT) | ilvI | acetolactate synthase III, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (8/4); new base C (6/8); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGG > W3110S.gb/85905‑86023 | ttccattccaTTAGTTGTCCTTTCCGGGCAGGTAGCGATCTCGTTGATAGGTTACGATGCCTTTCAgg < 1:2961252/68‑1 (MQ=255) ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGc < 1:839530/68‑1 (MQ=255) ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGc < 1:646607/68‑1 (MQ=255) ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGc < 1:2091749/68‑1 (MQ=255) ttccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGc < 1:2509148/68‑1 (MQ=255) acattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGAc > 1:1106262/2‑69 (MQ=255) ttGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACAtggtg > 1:877843/1‑68 (MQ=255) tGTCCTTTCCGGGCATGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTggg < 1:2654841/69‑1 (MQ=255) tCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCt < 1:2761902/40‑1 (MQ=255) tCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCt < 1:974356/40‑1 (MQ=255) tCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACAtgg < 1:2380248/58‑1 (MQ=255) tCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACAtgg < 1:2194306/58‑1 (MQ=255) ccGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCt > 1:2920029/1‑39 (MQ=255) ccGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCt > 1:1790172/1‑39 (MQ=255) aGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGa < 1:3448981/42‑1 (MQ=255) aGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGa < 1:2819042/42‑1 (MQ=255) aGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtgg > 1:2088910/1‑68 (MQ=255) aGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtgg > 1:2993997/1‑68 (MQ=255) aGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtgg > 1:1883814/1‑68 (MQ=255) aGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtgg > 1:690683/1‑68 (MQ=255) aGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtgg > 1:2697178/1‑68 (MQ=255) gACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGtt > 1:576314/1‑67 (MQ=255) gACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTa > 1:2013748/1‑68 (MQ=255) gACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTa < 1:2623530/68‑1 (MQ=255) tGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAg < 1:1985103/67‑1 (MQ=255) tAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAg > 1:495187/1‑64 (MQ=255) tAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTc > 1:1430280/1‑69 (MQ=255) tACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTgg > 1:815927/1‑67 (MQ=255) tACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACAGGTGGTTAAACACAGTTTTCTgg > 1:2398716/1‑67 (MQ=255) aaCGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCt > 1:3035177/2‑65 (MQ=255) | TTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGG > W3110S.gb/85905‑86023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |