| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,773,435 | T→C | 77.1% | L488L (TTG→CTG) | yfjW → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,773,435 | 0 | T | C | 77.1% | 123.9 / 33.6 | 61 | L488L (TTG→CTG) | yfjW | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (2/12); new base C (18/29); total (20/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
TTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTT > W3110S.gb/2773369‑2773500 | tttAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:2938315/69‑1 (MQ=255)tttAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:1882522/69‑1 (MQ=255)tttAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:2584103/69‑1 (MQ=255) tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTa < 1:3029993/69‑1 (MQ=255) tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTa < 1:3492264/69‑1 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATgg > 1:1306124/1‑67 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATgg > 1:2734695/1‑67 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATgg > 1:1659475/1‑67 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATgg > 1:3088037/1‑67 (MQ=255) aaCGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATgg > 1:1987211/1‑65 (MQ=255) aaCGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGa > 1:1524728/1‑66 (MQ=255) aaCGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGa > 1:3603445/1‑66 (MQ=255) aaCGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGa > 1:2020042/1‑66 (MQ=255) aCGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:2765577/59‑1 (MQ=255) gggAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGAc > 1:1960779/1‑64 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:3680896/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:3518098/54‑1 (MQ=255) aaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGt < 1:2604441/54‑1 (MQ=255) aaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGt < 1:2759155/54‑1 (MQ=255) aTTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATaaa > 1:759659/1‑68 (MQ=255) tttGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGTAGTCTg < 1:8440/49‑1 (MQ=255) tttGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa < 1:1478391/51‑1 (MQ=255) ttGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg > 1:2961680/1‑48 (MQ=255) ttGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg > 1:2595873/1‑48 (MQ=255) ttGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg > 1:1457784/1‑48 (MQ=255) ttGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg > 1:1381186/1‑48 (MQ=255) tGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:2339853/47‑1 (MQ=255) tGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:2511821/47‑1 (MQ=255) tGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:206949/47‑1 (MQ=255) tGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:184287/47‑1 (MQ=255) tGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:708338/47‑1 (MQ=255) tGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:1316245/47‑1 (MQ=255) tGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:382055/47‑1 (MQ=255) gTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCaaaaa < 1:3650182/69‑1 (MQ=255) tAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCaaaaaa < 1:2423633/68‑1 (MQ=255) gCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCaa < 1:1451524/69‑1 (MQ=255) gCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaa < 1:1724358/69‑1 (MQ=255) gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAata < 1:306027/69‑1 (MQ=255) gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAata < 1:520746/69‑1 (MQ=255) aaTCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatat < 1:2544652/69‑1 (MQ=255) aTCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatata < 1:2633674/69‑1 (MQ=255) aTCGCAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAata < 1:785020/67‑1 (MQ=255) tattattTCTGAAGGGAGTTTGTATGGACTAGTtaata < 1:922356/38‑1 (MQ=255) tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCaaaaaa < 1:3230654/49‑1 (MQ=255) tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCaaaaaa < 1:3238107/49‑1 (MQ=255) tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCaaaaaa < 1:796385/49‑1 (MQ=255) tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatatat > 1:604809/1‑61 (MQ=255) tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatatat > 1:166518/1‑61 (MQ=255) tattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatatat > 1:1629515/1‑61 (MQ=255) attattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGcac > 1:1842213/1‑65 (MQ=255) attattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGcac > 1:400905/1‑65 (MQ=255) attattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcagca < 1:3552394/69‑1 (MQ=255) tCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCtt < 1:1288576/69‑1 (MQ=255) cTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTg > 1:2785976/1‑69 (MQ=255) gggAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGtt < 1:2639406/66‑1 (MQ=255) gggAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGtt < 1:148082/66‑1 (MQ=255) gggAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGtt < 1:991559/66‑1 (MQ=255) ggAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTttg < 1:1394944/69‑1 (MQ=255) aGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTttgtt < 1:2449733/69‑1 (MQ=255) aGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTttgtt < 1:1839774/69‑1 (MQ=255) aGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTttgtt < 1:117513/69‑1 (MQ=255) | TTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTT > W3110S.gb/2773369‑2773500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |