| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,794,102 | G→A | 75.0% | L398L (TTG→TTA) | gabP → | gamma‑aminobutyrate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,794,102 | 0 | G | A | 75.0% | 117.0 / 44.7 | 68 | L398L (TTG→TTA) | gabP | gamma‑aminobutyrate transporter |
| Reads supporting (aligned to +/- strand): ref base G (6/11); new base A (25/26); total (31/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01 | |||||||||||
TCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTT > W3110S.gb/2794038‑2794161 | tCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGa < 1:1041419/68‑1 (MQ=255) cATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGaa < 1:308779/68‑1 (MQ=255) aTCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCg < 1:193842/65‑1 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTgg > 1:3631986/1‑69 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTgg > 1:3336315/1‑69 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTgg > 1:1484086/1‑69 (MQ=255) cttttCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCt < 1:2875755/67‑1 (MQ=255) cGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCt < 1:369766/69‑1 (MQ=255) cGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCt < 1:2016547/69‑1 (MQ=255) gTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtt < 1:3512147/69‑1 (MQ=255) gTTTCACAGTTGCGGATGCGCAAAATTCTGCGACCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCtt < 1:1915458/69‑1 (MQ=255) ttCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTa < 1:1088019/69‑1 (MQ=255) ttCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTa < 1:2766079/69‑1 (MQ=255) ttCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTa < 1:2552407/69‑1 (MQ=255) tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTAt > 1:936607/1‑69 (MQ=255) tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTAt < 1:554232/69‑1 (MQ=255) tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTAt > 1:1109784/1‑69 (MQ=255) acaGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATc < 1:3239328/68‑1 (MQ=255) gCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGc > 1:3371104/1‑56 (MQ=255) gCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGc > 1:2427180/1‑56 (MQ=255) gCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCtt < 1:3474199/58‑1 (MQ=255) gCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCtt < 1:76967/58‑1 (MQ=255) gCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCtt < 1:2688126/58‑1 (MQ=255) gCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCt > 1:2198905/1‑69 (MQ=255) gCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCt > 1:1529404/1‑69 (MQ=255) ggATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTc < 1:1919147/68‑1 (MQ=255) ggATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTc < 1:1579422/68‑1 (MQ=255) ggATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTc < 1:1642780/68‑1 (MQ=255) ggATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTc < 1:827689/68‑1 (MQ=255) gATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCtt < 1:1194893/55‑1 (MQ=255) tGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCtt < 1:3602813/53‑1 (MQ=255) tGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCtt < 1:212039/53‑1 (MQ=255) tGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCtt < 1:2643026/53‑1 (MQ=255) gcgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTg > 1:2892376/1‑69 (MQ=255) gcgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTg > 1:1884192/1‑69 (MQ=255) gcgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGc < 1:380423/50‑1 (MQ=255) gcgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGc < 1:2704685/50‑1 (MQ=255) gcgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGc < 1:3435605/50‑1 (MQ=255) gcgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGc < 1:1489496/50‑1 (MQ=255) gcgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGc < 1:2875764/50‑1 (MQ=255) gcgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTg > 1:1755225/1‑69 (MQ=255) cgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTgg > 1:2885837/1‑60 (MQ=255) cgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCt > 1:3491636/1‑62 (MQ=255) gcAAAATTCTGCGAGCAGAAGGAAGCGAATTTCGCTTACGAATGTGGGtt < 1:3175823/50‑1 (MQ=255) aTTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTc > 1:2625723/1‑69 (MQ=255) aTTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTc > 1:1500585/1‑69 (MQ=255) aGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:668672/69‑1 (MQ=255) aGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:1170046/69‑1 (MQ=255) cAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:2948160/49‑1 (MQ=255) aGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttat < 1:195315/69‑1 (MQ=255) aGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttat < 1:873559/69‑1 (MQ=255) aGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttat < 1:1983171/69‑1 (MQ=255) aGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATAGGCTttat < 1:1254576/69‑1 (MQ=255) ggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTAcc > 1:1983045/1‑69 (MQ=255) ggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTAcc > 1:2134716/1‑69 (MQ=255) ggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTAcc > 1:3189648/1‑69 (MQ=255) ggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTAcc > 1:664326/1‑69 (MQ=255) ggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTAcc > 1:1156509/1‑69 (MQ=255) ggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTAcc > 1:1557874/1‑69 (MQ=255) agCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGc > 1:1595936/1‑46 (MQ=255) gAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTc > 1:512691/1‑36 (MQ=255) gAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTc > 1:1191485/1‑36 (MQ=255) gAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTc > 1:1895680/1‑36 (MQ=255) gAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTc > 1:852128/1‑36 (MQ=255) aTTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGtt > 1:2351820/1‑68 (MQ=255) aTTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGtt > 1:1537246/1‑68 (MQ=255) aTTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGtt > 1:2222416/1‑68 (MQ=255) ttCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCt > 1:3176390/1‑51 (MQ=255) | TCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTT > W3110S.gb/2794038‑2794161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |