| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,821,480 | T→C | 59.4% | T316A (ACC→GCC) | recA ← | DNA strand exchange and recombination protein with protease and nuclease activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,821,480 | 0 | T | C | 59.4% | 21.8 / 31.2 | 32 | T316A (ACC→GCC) | recA | DNA strand exchange and recombination protein with protease and nuclease activity |
| Reads supporting (aligned to +/- strand): ref base T (5/8); new base C (8/11); total (13/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTT > W3110S.gb/2821421‑2821542 | cGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGtt < 1:1739271/69‑1 (MQ=255)cGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGtt < 1:1823905/69‑1 (MQ=255)cGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGtt < 1:3007318/69‑1 (MQ=255) gTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTa < 1:429344/69‑1 (MQ=255) gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATc > 1:2145275/1‑68 (MQ=255) cGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGtt < 1:3408118/60‑1 (MQ=255) gTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCa > 1:3615127/1‑65 (MQ=255) gcagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAg > 1:16059/1‑62 (MQ=255) gcagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCg > 1:1966398/1‑69 (MQ=255) gcagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCg > 1:2841512/1‑69 (MQ=255) cagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCTGGTTATCTTTCAGCCAGGCAGTCg < 1:1192784/68‑1 (MQ=255) cagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCg < 1:787499/68‑1 (MQ=255) cagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCg < 1:200494/68‑1 (MQ=255) cagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCg < 1:2352030/68‑1 (MQ=255) cagcaACTCACGTACTTTCTTCTCGATCTATTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCg < 1:3215392/68‑1 (MQ=255) agcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCa < 1:3212892/69‑1 (MQ=255) agcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCa < 1:3607343/69‑1 (MQ=255) agcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCa < 1:2849476/69‑1 (MQ=255) caACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCAtt < 1:815731/69‑1 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCg > 1:3298157/1‑64 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTATCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTc > 1:3370437/1‑69 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:1732669/69‑1 (MQ=255) tCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc > 1:707772/1‑68 (MQ=255) cGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAGCCTGACCGa < 1:921391/66‑1 (MQ=255) tctcTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtcttct > 1:93808/1‑69 (MQ=255) tctcTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtcttct > 1:665537/1‑69 (MQ=255) tctcTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtctt < 1:714814/67‑1 (MQ=255) tCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtcttct < 1:2706105/63‑1 (MQ=255) tCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTtctc < 1:2218037/64‑1 (MQ=255) tCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCtt > 1:2885503/1‑69 (MQ=255) tCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCtt > 1:2881089/1‑69 (MQ=255) tCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCtt > 1:2058029/1‑69 (MQ=255) | CGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTT > W3110S.gb/2821421‑2821542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |