| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,821,628 | G→A | 60.7% | G266G (GGC→GGT) | recA ← | DNA strand exchange and recombination protein with protease and nuclease activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,821,628 | 0 | G | A | 60.7% | 17.2 / 30.1 | 30 | G266G (GGC→GGT) | recA | DNA strand exchange and recombination protein with protease and nuclease activity |
| Reads supporting (aligned to +/- strand): ref base G (5/6); new base A (7/11); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAC > W3110S.gb/2821566‑2821693 | ttCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCACCGTa < 1:2234499/68‑1 (MQ=255)ttCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCACCGTa < 1:2157240/68‑1 (MQ=255)ttCTCGATCAGCTTCTATTTTACGCCCAGGTCAACGAGTTCGCCGTAGAAGTTGATACCTTCACCGTa < 1:1550244/68‑1 (MQ=255) ctcGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTagag > 1:3652768/1‑69 (MQ=255) ctcGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTagag > 1:1785274/1‑69 (MQ=255) cAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGttt < 1:3658067/61‑1 (MQ=255) cAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGttt < 1:2775458/61‑1 (MQ=255) cAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTa < 1:960753/62‑1 (MQ=255) cAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTa < 1:1198596/62‑1 (MQ=255) cAACCAGTTCGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACgg > 1:2240508/1‑67 (MQ=255) cAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGc > 1:2242567/1‑69 (MQ=255) cAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGc > 1:1000653/1‑69 (MQ=255) gTTCGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGa < 1:1839542/69‑1 (MQ=255) gTTCGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGa < 1:1284703/69‑1 (MQ=255) gTTCGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGa < 1:1797610/69‑1 (MQ=255) gTTCGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGa < 1:3423857/69‑1 (MQ=255) gAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTa < 1:1137615/46‑1 (MQ=255) aGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGttcttc < 1:2866766/68‑1 (MQ=255) gTTGATACCTTCGCGGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTcac > 1:3409387/1‑69 (MQ=255) gTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGAttt < 1:2768993/59‑1 (MQ=255) gTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGAttt < 1:2862242/59‑1 (MQ=255) gTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGAttt < 1:114604/59‑1 (MQ=255) ttGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTcacc < 1:830276/69‑1 (MQ=255) tGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTcacca > 1:549024/1‑69 (MQ=255) tGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTcacca > 1:2337516/1‑69 (MQ=255) tGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTcacca > 1:3619567/1‑69 (MQ=255) ccTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTc > 1:2427867/1‑69 (MQ=255) ccTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTc > 1:986370/1‑69 (MQ=255) ttCTCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAc > 1:1111238/1‑69 (MQ=255) ttCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGttcttc > 1:88585/1‑58 (MQ=255) | TTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAC > W3110S.gb/2821566‑2821693 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |