| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,840,761 | A→G | 74.1% | E372E (GAA→GAG) | ascB → | cryptic 6‑phospho‑beta‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,840,761 | 0 | A | G | 74.1% | 98.3 / 32.6 | 54 | E372E (GAA→GAG) | ascB | cryptic 6‑phospho‑beta‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base A (9/5); new base G (18/22); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCA > W3110S.gb/2840698‑2840825 | cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACgg > 1:1084067/1‑69 (MQ=255)cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACgg > 1:701528/1‑69 (MQ=255)cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACgg > 1:267648/1‑69 (MQ=255)cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACgg > 1:2304471/1‑69 (MQ=255)cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACg > 1:2498185/1‑68 (MQ=255) tCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGcc < 1:2576093/68‑1 (MQ=255) tCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGc < 1:970255/67‑1 (MQ=255) tATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTggg < 1:3142693/66‑1 (MQ=255) aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGgcgc > 1:643306/1‑65 (MQ=255) aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGgcgc > 1:2939102/1‑65 (MQ=255) aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGgcgc > 1:315845/1‑65 (MQ=255) aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGgcgc > 1:1344186/1‑65 (MQ=255) aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGgcg > 1:3698601/1‑64 (MQ=255) aaTATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGa > 1:2428738/1‑68 (MQ=255) atgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAttt < 1:1971213/69‑1 (MQ=255) atgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAttt < 1:2769745/69‑1 (MQ=255) atgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAttt < 1:3153077/69‑1 (MQ=255) atgatgTACGAGCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAttt < 1:1987002/69‑1 (MQ=38) tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:3426267/69‑1 (MQ=255) tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:3097541/1‑69 (MQ=255) tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:2857910/1‑69 (MQ=255) tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:2801216/69‑1 (MQ=255) cGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGc < 1:80668/69‑1 (MQ=255) tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:3322219/68‑1 (MQ=255) tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:3624331/68‑1 (MQ=255) tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:1057611/68‑1 (MQ=255) aTCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:3384601/1‑59 (MQ=255) aTCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag > 1:2467669/1‑68 (MQ=255) aTCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag > 1:527713/1‑68 (MQ=255) aGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:2634018/68‑1 (MQ=255) aGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:2073516/68‑1 (MQ=255) aGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:2139206/56‑1 (MQ=255) aGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:352871/56‑1 (MQ=255) gCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacg > 1:3449232/1‑68 (MQ=255) ccGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgac < 1:906316/69‑1 (MQ=255) ccGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgac < 1:2130830/69‑1 (MQ=255) ccGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgac < 1:2353751/69‑1 (MQ=255) tGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACt < 1:492809/69‑1 (MQ=255) tGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACt < 1:3015397/69‑1 (MQ=255) tGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACt < 1:1407015/69‑1 (MQ=255) tGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACt < 1:3459354/69‑1 (MQ=255) gTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATg > 1:1761517/1‑35 (MQ=255) gTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATg > 1:1477748/1‑35 (MQ=255) gTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATg > 1:3134361/1‑35 (MQ=255) tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAac > 1:1098086/1‑58 (MQ=255) tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACt > 1:2933501/1‑65 (MQ=255) tCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCg > 1:3649382/1‑69 (MQ=255) tCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCg > 1:2444769/1‑69 (MQ=255) tggtggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGc < 1:1150748/68‑1 (MQ=255) tggtggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTACCAATGGCGAGATTAACGACTACTATCGc < 1:3059795/68‑1 (MQ=255) ggtggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:1177663/68‑1 (MQ=255) ggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCa > 1:3141268/1‑68 (MQ=255) gAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:603077/1‑36 (MQ=255) gAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:930228/1‑36 (MQ=255) | CGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCA > W3110S.gb/2840698‑2840825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |