| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,841,727 | C→T | 54.8% | A126T (GCC→ACC) | hycH ← | protein required for maturation of hydrogenase 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,841,727 | 0 | C | T | 54.8% | 21.7 / 130.8 | 93 | A126T (GCC→ACC) | hycH | protein required for maturation of hydrogenase 3 |
| Reads supporting (aligned to +/- strand): ref base C (24/18); new base T (26/25); total (50/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCG > W3110S.gb/2841664‑2841789 | catGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGtttt > 1:302179/1‑69 (MQ=255)catGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGtttt > 1:532169/1‑69 (MQ=255)catGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGtttt > 1:484952/1‑69 (MQ=255)catGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGttt < 1:2757467/68‑1 (MQ=255)catGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGttt < 1:3393703/68‑1 (MQ=255)catGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGttt < 1:1484990/68‑1 (MQ=255)catGCTATTGACAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGttt < 1:3489222/68‑1 (MQ=255) gCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:2911162/69‑1 (MQ=255) gCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:3527236/69‑1 (MQ=255) gCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:1445119/69‑1 (MQ=255) aTTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgat < 1:2379742/69‑1 (MQ=255) ttGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgatg > 1:24397/1‑69 (MQ=255) ttGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgatg > 1:2962132/1‑69 (MQ=255) gCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgatga > 1:61079/1‑68 (MQ=255) gCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGaa > 1:733340/1‑69 (MQ=255) gCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGaa > 1:2509617/1‑69 (MQ=255) gCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGaa > 1:347834/1‑69 (MQ=255) ccAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAat > 1:3273318/1‑69 (MQ=255) aaCACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAatat > 1:2004509/1‑69 (MQ=255) acacAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAatat < 1:142729/68‑1 (MQ=255) acacAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAatat > 1:370076/1‑68 (MQ=255) acacAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATc > 1:1983339/1‑69 (MQ=255) cacaGAGTAAAACGTCGGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCa < 1:392372/69‑1 (MQ=255) caGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCa > 1:3354281/1‑67 (MQ=255) caGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCa > 1:792146/1‑67 (MQ=255) caGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCa > 1:94543/1‑67 (MQ=255) caGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCa > 1:2817283/1‑67 (MQ=255) agagTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgatg < 1:1078368/59‑1 (MQ=255) agagTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgatg < 1:1861285/59‑1 (MQ=255) agagTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgatg < 1:1056384/59‑1 (MQ=255) agagTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgatg < 1:694695/59‑1 (MQ=255) agagTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgatg < 1:2429563/59‑1 (MQ=255) tAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGaa > 1:788155/1‑57 (MQ=255) tAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATgcagca > 1:2964859/1‑69 (MQ=255) tAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATgcagca > 1:2800242/1‑69 (MQ=255) tAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATgcagca > 1:1946989/1‑69 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATa < 1:2984685/69‑1 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATa < 1:3647688/69‑1 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATa < 1:1897275/69‑1 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATa > 1:1769044/1‑69 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATa > 1:1213090/1‑69 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatg > 1:1436783/1‑53 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatg > 1:1357894/1‑53 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatg > 1:3705723/1‑53 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatg > 1:3556882/1‑53 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatg > 1:2581043/1‑53 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatg > 1:2321150/1‑53 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATa > 1:3703410/1‑69 (MQ=255) cGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:1956585/69‑1 (MQ=255) gtcagtcaCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTa < 1:1711796/69‑1 (MQ=255) gtcagtcaCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTa < 1:2571875/69‑1 (MQ=255) caCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATc > 1:3132183/1‑49 (MQ=255) gCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:1324915/69‑1 (MQ=255) gCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:119729/69‑1 (MQ=255) gCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:1155998/69‑1 (MQ=255) ctcctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACtt > 1:2557674/1‑54 (MQ=255) ctcctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTc < 1:2997945/69‑1 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg > 1:1662857/1‑63 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTcc > 1:2509828/1‑69 (MQ=255) cACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCAt < 1:2020955/67‑1 (MQ=255) cACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACtt < 1:1291234/49‑1 (MQ=255) cACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACtt < 1:2820140/49‑1 (MQ=255) cACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACtt < 1:1067815/49‑1 (MQ=255) aCCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:205083/58‑1 (MQ=255) aCCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTcc > 1:3139184/1‑69 (MQ=255) aCCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTcc > 1:2600807/1‑69 (MQ=255) cATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTcc < 1:1258154/67‑1 (MQ=255) cATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCct < 1:3624725/68‑1 (MQ=255) aTCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCctgc > 1:391374/1‑69 (MQ=255) aTCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCctgc > 1:499281/1‑69 (MQ=255) aTCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCctgc > 1:3606929/1‑69 (MQ=255) cAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCctgctg > 1:749013/1‑69 (MQ=255) aaaTAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAg < 1:2332701/47‑1 (MQ=255) aaaTAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAg < 1:3621838/47‑1 (MQ=255) aaaTAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAg < 1:226033/47‑1 (MQ=255) aaaTAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTGctgctg > 1:827460/1‑68 (MQ=255) aaaTAGATGGCGTTTTCCTGATGAATATCATgcag < 1:317888/35‑1 (MQ=255) aaaTAGATGGCGTTTTCCTGATGAATATCATgcag < 1:1352372/35‑1 (MQ=255) aaaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACtt > 1:2028607/1‑42 (MQ=255) aaaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACtt > 1:3691091/1‑42 (MQ=255) aaaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACtt > 1:2232280/1‑42 (MQ=255) tAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCctgctgctg > 1:826741/1‑68 (MQ=255) tAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCctgctgct > 1:2288343/1‑67 (MQ=255) aGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAg > 1:3712357/1‑43 (MQ=255) aGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAg > 1:2355576/1‑43 (MQ=255) aGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCg < 1:3175783/69‑1 (MQ=255) aGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCg < 1:3138346/69‑1 (MQ=255) aGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCg < 1:450383/69‑1 (MQ=255) aGATGGTGTTTTCCTGATGAATATCATGCAGCATAATTAACAg > 1:283079/1‑43 (MQ=38) gATGGCGTTTTCCTGATGAATATCATGCAGCATACtt < 1:1052429/37‑1 (MQ=255) gATGGCGTTTTCCTGATGAATATCATGCAGCATACtt < 1:2188848/37‑1 (MQ=255) aTGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCg < 1:2479261/67‑1 (MQ=255) ggCGTTTTCCTGATGAATATCATGCAGCATACTTAACAg > 1:973496/1‑39 (MQ=255) | CATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCG > W3110S.gb/2841664‑2841789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |