| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,860,110 | G→A | 71.7% | V9I (GTC→ATC) | ygbJ → | predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,860,110 | 0 | G | A | 71.7% | 76.1 / 40.3 | 53 | V9I (GTC→ATC) | ygbJ | predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (3/12); new base A (16/22); total (19/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
ATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTC > W3110S.gb/2860046‑2860173 | atatCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCgg < 1:2674627/69‑1 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTAt < 1:216781/69‑1 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTAt < 1:1438138/69‑1 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTAt < 1:1308281/69‑1 (MQ=255) caaacaaaATATCACTTAATTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATc > 1:3261217/1‑69 (MQ=255) caaacaaaATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTAt > 1:2007303/1‑68 (MQ=255) caaacaaaATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATc > 1:1014298/1‑69 (MQ=255) aaaaTATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTgg < 1:1476686/69‑1 (MQ=255) aaaaTATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTgg < 1:3234693/69‑1 (MQ=255) aaaaTATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTgg < 1:3679906/69‑1 (MQ=255) atatCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCtt < 1:3136618/69‑1 (MQ=255) tCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAgg > 1:3064124/1‑69 (MQ=255) tCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAgg > 1:979918/1‑69 (MQ=255) tCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAgg > 1:2114317/1‑69 (MQ=255) ttAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTc < 1:2811605/68‑1 (MQ=255) ttAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTc < 1:1159137/68‑1 (MQ=255) aaaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCtt < 1:393186/60‑1 (MQ=255) aaaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCtt < 1:2328751/60‑1 (MQ=255) ttAACTAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:1231158/69‑1 (MQ=255) ttAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCaatggg < 1:2128797/69‑1 (MQ=255) ttAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCaatggg < 1:1528433/69‑1 (MQ=255) ttAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCaatggg < 1:2601448/69‑1 (MQ=255) ttAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:997361/69‑1 (MQ=255) ttAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:1962099/69‑1 (MQ=255) aGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatgggaatggg < 1:2839341/69‑1 (MQ=255) ctgATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGagcagc < 1:2197001/66‑1 (MQ=255) tGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGt < 1:2768642/69‑1 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCt > 1:2997320/1‑38 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCt > 1:1961090/1‑38 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTa < 1:317535/40‑1 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTa < 1:1259474/40‑1 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTa < 1:1600936/40‑1 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTa < 1:962269/40‑1 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTa < 1:201045/40‑1 (MQ=255) gAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTc > 1:3519594/1‑45 (MQ=255) gAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGt > 1:3033205/1‑68 (MQ=255) aaaaCGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAg > 1:3311824/1‑40 (MQ=255) aaaaCGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg > 1:3328200/1‑50 (MQ=255) aaaaCGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatgg > 1:2374767/1‑49 (MQ=255) aaaaCGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa < 1:1144012/69‑1 (MQ=255) aaCGTGATCTGAGTTTCATATCGGTATCGTTGGCTTa < 1:2345842/37‑1 (MQ=37) aaCGGGCTCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa > 1:320772/1‑67 (MQ=255) aaCGGGATCTGAGTTTCATATCGGTATCGTTGGCTTa < 1:290432/37‑1 (MQ=255) aaCGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCata > 1:1195317/1‑69 (MQ=255) aaCGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa > 1:2526466/1‑67 (MQ=255) cGGTATCTGAGTTTCATATCGTTATCGTTGGCTTa < 1:1689433/35‑1 (MQ=25) cGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGagc > 1:2743757/1‑55 (MQ=255) cGGGATCTGAGTTTCATATCTGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGt < 1:2268797/63‑1 (MQ=255) cTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:2861290/40‑1 (MQ=255) cTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:994617/40‑1 (MQ=255) tGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa < 1:1764715/58‑1 (MQ=255) tCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTc > 1:3305533/1‑68 (MQ=255) tCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTc > 1:1162920/1‑68 (MQ=255) | ATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTC > W3110S.gb/2860046‑2860173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |