| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,866,764 | G→A | 7.3% | Q216* (CAG→TAG) | nlpD ← | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,866,764 | 0 | G | A | 7.3% | 174.5 / 3.4 | 55 | Q216* (CAG→TAG) | nlpD | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (22/29); new base A (2/2); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01 | |||||||||||
ACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCA > W3110S.gb/2866697‑2866831 | aCCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTg > 1:1948883/1‑68 (MQ=255) gTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTc < 1:1323385/68‑1 (MQ=255) aCAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCAcc > 1:448591/1‑68 (MQ=255) gcgcTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAg > 1:1013444/1‑69 (MQ=255) gcgcTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAg < 1:2764314/69‑1 (MQ=255) gcgcTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAg > 1:1784819/1‑69 (MQ=255) gtgACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTc > 1:1103770/1‑56 (MQ=255) gtgACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTc > 1:1128985/1‑56 (MQ=255) gtgACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTc < 1:3405864/56‑1 (MQ=255) gtgACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTc > 1:3276844/1‑56 (MQ=255) gtgACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTc < 1:3227746/56‑1 (MQ=255) aCCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAg < 1:89885/67‑1 (MQ=255) aCCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAg < 1:2295499/67‑1 (MQ=255) aCCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGa < 1:1657741/68‑1 (MQ=255) gTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTATTCACCCGAAGACTCAGAATa > 1:933373/1‑68 (MQ=255) ggTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGt < 1:1749308/69‑1 (MQ=255) ggTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGt < 1:1395470/69‑1 (MQ=255) ggTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGt < 1:645690/69‑1 (MQ=255) ggTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGt < 1:2258769/69‑1 (MQ=255) gTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGt < 1:147782/68‑1 (MQ=255) gTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTa > 1:2178826/1‑69 (MQ=255) gTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTa < 1:1840051/69‑1 (MQ=255) gTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTa > 1:462736/1‑69 (MQ=255) gTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTa < 1:2550914/69‑1 (MQ=255) gTCGCAGTTGGCTTGTTGTTCGGCAACATTCTGTTAGCACTCTATTCACCCGAAGACTCAGAATACGTa > 1:1344450/1‑69 (MQ=255) gCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAg < 1:837680/58‑1 (MQ=255) gCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAg < 1:1688916/58‑1 (MQ=255) gCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAg < 1:3641410/58‑1 (MQ=255) gCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAAtt > 1:1717466/1‑69 (MQ=255) gCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAAtt > 1:3107611/1‑69 (MQ=255) ggCTTGTTGTTCGGCAACATTTTGTTAGCACTCTg > 1:2196710/1‑35 (MQ=255) ggCTTGTTGTTCGGCAACATTTTGTTAGCACTCTg > 1:1419550/1‑35 (MQ=255) ggCTTGTTGTTCGGCAACATTTTGTTAGCACTCTg > 1:90853/1‑35 (MQ=255) ggCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGt > 1:1291478/1‑69 (MQ=255) ttgttgTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGa < 1:1630042/50‑1 (MQ=255) ttgttgTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGc < 1:2655644/69‑1 (MQ=255) ttgttgTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGc < 1:249688/69‑1 (MQ=255) ttgttgTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGc < 1:2447684/69‑1 (MQ=255) tgttgtTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAg < 1:283647/48‑1 (MQ=255) tgttgtTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAg < 1:3089092/48‑1 (MQ=255) gttCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGc < 1:177053/69‑1 (MQ=255) gttCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGc < 1:322490/69‑1 (MQ=255) aTTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGc < 1:2259803/59‑1 (MQ=255) aTTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGc > 1:385934/1‑59 (MQ=255) aTTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGc > 1:69782/1‑59 (MQ=255) tttGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACgg > 1:3111429/1‑68 (MQ=255) ttGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGt > 1:3556980/1‑68 (MQ=255) ttGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGt > 1:3004756/1‑68 (MQ=255) ttAGCACTCTATTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGa < 1:1105686/68‑1 (MQ=255) ttAGCACTCTATTCACCCGAAGACTCAGAATACGTAATTGTCGGTGGCGACGCAACAGCAACGGTGGa < 1:2396890/68‑1 (MQ=255) tAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGaa < 1:3436327/68‑1 (MQ=255) gCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAAttt > 1:789840/1‑69 (MQ=255) aCTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAAttt < 1:843238/67‑1 (MQ=255) tctGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTtgtg < 1:2264317/69‑1 (MQ=255) ctGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGc > 1:2665694/1‑69 (MQ=255) ctGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGc > 1:3684394/1‑69 (MQ=255) ctGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGc > 1:1691274/1‑69 (MQ=255) tGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCa > 1:2483909/1‑69 (MQ=255) tGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCa > 1:734516/1‑69 (MQ=255) | ACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCA > W3110S.gb/2866697‑2866831 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |