| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,867,123 | G→A | 69.2% | P96L (CCG→CTG) | nlpD ← | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,867,123 | 0 | G | A | 69.2% | 65.8 / 43.7 | 52 | P96L (CCG→CTG) | nlpD | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (9/7); new base A (16/20); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
TAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATT > W3110S.gb/2867057‑2867179 | taACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgc > 1:173228/1‑69 (MQ=255)taACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgc > 1:1996091/1‑69 (MQ=255) aCTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGgct < 1:1322393/68‑1 (MQ=255) aCTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGgct < 1:812811/68‑1 (MQ=255) aCTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgctg > 1:1357402/1‑69 (MQ=255) aCTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgctg > 1:2453408/1‑69 (MQ=255) aCTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAgctg > 1:390206/1‑69 (MQ=255) gCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGctgctg < 1:262226/69‑1 (MQ=255) tttCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:2424959/69‑1 (MQ=255) tttCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:3681974/69‑1 (MQ=255) ttCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:1983710/68‑1 (MQ=255) gAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACt < 1:2870784/68‑1 (MQ=255) aTGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGc > 1:1832583/1‑69 (MQ=255) gTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTg < 1:475356/69‑1 (MQ=255) aCTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGc > 1:3411024/1‑62 (MQ=255) aCTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGc > 1:95057/1‑62 (MQ=255) aCTGACCGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGc > 1:906767/1‑62 (MQ=255) cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCActggctg > 1:113742/1‑69 (MQ=255) cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCATCTGCTGAGCTACTGGCTGCActggctg < 1:3320427/69‑1 (MQ=255) cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCActggctg < 1:3145309/69‑1 (MQ=255) cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCActggctg > 1:423360/1‑69 (MQ=255) gACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCActgtctgg < 1:3499825/68‑1 (MQ=255) gACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGa > 1:2931028/1‑69 (MQ=255) gACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGa > 1:1308961/1‑69 (MQ=255) gACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGa > 1:761646/1‑69 (MQ=255) gACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGa > 1:716628/1‑69 (MQ=255) gACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGa > 1:1984287/1‑69 (MQ=255) gACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGa > 1:2394188/1‑69 (MQ=255) ttATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:325734/44‑1 (MQ=255) ttATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:2606671/44‑1 (MQ=255) ttATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:1637458/44‑1 (MQ=255) ttATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:3132804/44‑1 (MQ=255) ttATAGACGATGCGTACGTTTTCCATCTGTACCAGCTGCTGAGc < 1:3022644/44‑1 (MQ=38) tataGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACtc > 1:137116/1‑47 (MQ=255) tataGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTg > 1:328155/1‑48 (MQ=255) cGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGc > 1:1510964/1‑47 (MQ=255) cGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGc > 1:812225/1‑47 (MQ=255) cGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgttgtt < 1:3504596/69‑1 (MQ=255) cGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgttgtt < 1:395322/69‑1 (MQ=255) cGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgttgtt < 1:1632471/69‑1 (MQ=255) cGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgttgtt < 1:3203107/69‑1 (MQ=255) cGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgttgtt < 1:793932/69‑1 (MQ=255) aTGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGttgttg > 1:935899/1‑68 (MQ=255) aTGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGttgttg > 1:841433/1‑68 (MQ=255) cGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAg < 1:2504333/63‑1 (MQ=255) cGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAg < 1:349595/63‑1 (MQ=255) cGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAg < 1:665956/63‑1 (MQ=255) ttttCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGa < 1:665770/69‑1 (MQ=255) ttCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGaa > 1:1351878/1‑68 (MQ=255) ttCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAAt > 1:2238559/1‑69 (MQ=255) tACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAAtt < 1:2946196/61‑1 (MQ=255) aCCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAAtt < 1:775429/60‑1 (MQ=255) | TAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATT > W3110S.gb/2867057‑2867179 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |