| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,875,612 | G→A | 28.6% | D126N (GAT→AAT) | iap → | aminopeptidase in alkaline phosphatase isozyme conversion |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,875,612 | 0 | G | A | 28.6% | 78.6 / 33.8 | 49 | D126N (GAT→AAT) | iap | aminopeptidase in alkaline phosphatase isozyme conversion |
| Reads supporting (aligned to +/- strand): ref base G (17/18); new base A (6/8); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTA > W3110S.gb/2875544‑2875680 | cTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCg < 1:2943336/69‑1 (MQ=255)cTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCg < 1:529477/69‑1 (MQ=255)cTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCg < 1:476449/69‑1 (MQ=255) cATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAAt > 1:558267/1‑69 (MQ=255) cATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAAt > 1:178671/1‑69 (MQ=255) aTGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGAt < 1:1518897/68‑1 (MQ=255) gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:1690623/69‑1 (MQ=255) gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:3179284/69‑1 (MQ=255) gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:1917766/69‑1 (MQ=255) gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:2256365/69‑1 (MQ=255) gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:2664622/69‑1 (MQ=255) aaGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTg > 1:1880077/1‑69 (MQ=255) gcgcCGCAGCAGATCATCATTATGGCGCATCTTGATACTTACGCCCCGCTGAGCGa < 1:3337308/56‑1 (MQ=255) gcgcCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGa < 1:3617497/56‑1 (MQ=255) gcgcCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGa < 1:3073045/56‑1 (MQ=255) gccgcAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGc < 1:2523575/69‑1 (MQ=255) gccgcAGCAGATCATCATTATGGCGAATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGc < 1:3595151/69‑1 (MQ=255) atcatcATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTcggc < 1:3155090/69‑1 (MQ=255) tcatcaTTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTcggcg > 1:363657/1‑69 (MQ=255) catcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:1164571/46‑1 (MQ=255) atcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt > 1:2703660/1‑45 (MQ=255) catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAAtctc > 1:1808850/1‑61 (MQ=255) catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt > 1:3415699/1‑69 (MQ=255) catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt < 1:514859/69‑1 (MQ=255) catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt < 1:725746/69‑1 (MQ=255) catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt > 1:2011926/1‑69 (MQ=255) atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:3008096/1‑69 (MQ=255) atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:2833572/1‑69 (MQ=255) atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:75249/1‑69 (MQ=255) atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:667176/1‑69 (MQ=255) atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:1738/1‑69 (MQ=255) atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg > 1:1635746/1‑69 (MQ=255) aTGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGAc > 1:617040/1‑68 (MQ=255) aTGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGAc > 1:3360291/1‑68 (MQ=255) tCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg < 1:2900013/69‑1 (MQ=255) tCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg > 1:3303735/1‑69 (MQ=255) tCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg < 1:3450820/69‑1 (MQ=255) tCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg < 1:36648/69‑1 (MQ=255) tGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa > 1:243102/1‑69 (MQ=255) tGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa > 1:1219580/1‑69 (MQ=255) tACTTACGCCCCGCTGAGCGATGCTGACGCCGATTCCAATCTCGGCGGGCTGACGTTACAAGGAATg < 1:58640/67‑1 (MQ=255) tACGCCGCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatgat < 1:2643932/69‑1 (MQ=255) tACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatgat < 1:366503/69‑1 (MQ=255) tACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatgat < 1:868447/69‑1 (MQ=255) cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGTCTGACGTTACAAGGAATGgat > 1:1435277/1‑64 (MQ=255) cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat < 1:165721/64‑1 (MQ=255) cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat < 1:2828305/64‑1 (MQ=255) cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat > 1:3460001/1‑64 (MQ=255) cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat > 1:3670687/1‑64 (MQ=255) cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat < 1:2824026/64‑1 (MQ=255) cGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat > 1:2834733/1‑64 (MQ=255) cGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg < 1:937004/69‑1 (MQ=255) gATGCTGACGCCGATGCCAATCTCGGCGTGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTa < 1:377312/69‑1 (MQ=255) gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatga < 1:540027/53‑1 (MQ=255) gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatga < 1:1747703/53‑1 (MQ=255) gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatga < 1:2803764/53‑1 (MQ=255) gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTa < 1:1043418/69‑1 (MQ=255) gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTa < 1:3637460/69‑1 (MQ=255) gATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTa < 1:327702/69‑1 (MQ=255) | CTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTA > W3110S.gb/2875544‑2875680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |