| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,885,984 | C→T | 100% | intergenic (‑109/+250) | ygcB ← / ← cysH | conserved hypothetical protein, member of DEAD box family/3'‑phosphoadenosine 5'‑phosphosulfate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,885,984 | 0 | C | T | 85.1% | 167.6 / 21.7 | 67 | intergenic (‑109/+250) | ygcB/cysH | conserved hypothetical protein, member of DEAD box family/3'‑phosphoadenosine 5'‑phosphosulfate reductase |
| Reads supporting (aligned to +/- strand): ref base C (10/0); new base T (32/25); total (42/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCT > W3110S.gb/2885924‑2886052 | tttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAagaaga > 1:1168625/1‑69 (MQ=255)tttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAagaaga > 1:3587222/1‑69 (MQ=255) tttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTaaa > 1:2874149/1‑62 (MQ=255) tttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTaa > 1:1681853/1‑61 (MQ=255) tttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAagag > 1:1112659/1‑68 (MQ=255) gctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCg < 1:3581956/69‑1 (MQ=255) cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACCTTCGaa < 1:480809/68‑1 (MQ=255) cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGaa < 1:815402/68‑1 (MQ=255) cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGa < 1:2979922/67‑1 (MQ=255) cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGa < 1:1870292/67‑1 (MQ=255) cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAAt > 1:941593/1‑69 (MQ=255) aaTATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAaagaa > 1:811051/1‑48 (MQ=255) aaTATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAaagaa > 1:2139017/1‑48 (MQ=255) aaTATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTa > 1:1130231/1‑55 (MQ=255) aaTATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTa > 1:2007342/1‑55 (MQ=255) aaTATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGtt > 1:1690541/1‑69 (MQ=255) tatCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTg < 1:3191690/69‑1 (MQ=255) atCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTg < 1:1941771/68‑1 (MQ=255) tCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTg < 1:1245909/67‑1 (MQ=255) gAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCg < 1:2752518/56‑1 (MQ=255) gAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGttt > 1:3147197/1‑64 (MQ=255) gAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGttt > 1:3132458/1‑64 (MQ=255) gAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGttt < 1:1447047/64‑1 (MQ=255) gAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGttt < 1:2770111/64‑1 (MQ=255) gAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGttt > 1:2600523/1‑64 (MQ=255) gAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGttt < 1:236173/64‑1 (MQ=255) gAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGttt > 1:1587138/1‑64 (MQ=255) gAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGttt > 1:1603447/1‑64 (MQ=255) ttACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGa > 1:3490432/1‑69 (MQ=255) tACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGaa > 1:3720176/1‑69 (MQ=255) tACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGaa > 1:796997/1‑69 (MQ=255) aGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACg < 1:1395813/68‑1 (MQ=255) aGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACg < 1:1426293/68‑1 (MQ=255) aGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACg < 1:2918179/68‑1 (MQ=255) gTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACg < 1:1869044/67‑1 (MQ=255) atatACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGc > 1:716313/1‑69 (MQ=255) atatACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGc > 1:210720/1‑69 (MQ=255) ataCACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGc > 1:3351379/1‑67 (MQ=255) ataCACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGc > 1:3202422/1‑67 (MQ=255) taCACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGATTTGTTTGGGTTCGAACGCTGGCCTc > 1:1944121/1‑69 (MQ=255) taCACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTc > 1:2556216/1‑69 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTg > 1:1265921/1‑45 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAg > 1:1136463/1‑69 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAg < 1:3668820/69‑1 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAg > 1:1564156/1‑69 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAg > 1:2191455/1‑69 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAg < 1:2701896/69‑1 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAg > 1:273226/1‑69 (MQ=255) acaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAg < 1:1699100/68‑1 (MQ=255) acaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGACTCAg < 1:1616138/68‑1 (MQ=255) tGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGt > 1:3656294/1‑42 (MQ=255) tGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGtt > 1:2581193/1‑68 (MQ=255) tGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGtt > 1:3168123/1‑68 (MQ=255) tCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCAAACGCTGGCCTCAGGtt > 1:657709/1‑66 (MQ=255) tCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCAAACGCTGGCCTCAGGtt > 1:1890462/1‑66 (MQ=255) tCTTGCATCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGtt > 1:323590/1‑66 (MQ=255) cTTGCCTCCCTGTAAAGAAGAGACTAAGATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGAt > 1:692951/1‑68 (MQ=255) ttGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATa > 1:3317088/1‑68 (MQ=255) ccTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGa < 1:2554831/63‑1 (MQ=255) ccTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGa < 1:3445001/63‑1 (MQ=255) ccTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTGCGAACGCTGGCCTCAGGTTGa < 1:3385129/63‑1 (MQ=255) cTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa < 1:12442/66‑1 (MQ=255) cTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa < 1:2727646/66‑1 (MQ=255) tGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGc > 1:845981/1‑69 (MQ=255) gCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa > 1:2872421/1‑60 (MQ=255) gCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa > 1:3189592/1‑60 (MQ=255) gCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa > 1:2544159/1‑60 (MQ=255) cAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCt > 1:589635/1‑69 (MQ=255) cAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCt > 1:854416/1‑69 (MQ=255) | TTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCT > W3110S.gb/2885924‑2886052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |