| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,893,179 | A→C | 24.6% | I110L (ATT→CTT) | ygcP → | predicted anti‑terminator regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,893,179 | 0 | A | C | 24.6% | 49.6 / 44.2 | 53 | I110L (ATT→CTT) | ygcP | predicted anti‑terminator regulatory protein |
| Reads supporting (aligned to +/- strand): ref base A (23/17); new base C (9/4); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01 | |||||||||||
AGTACCAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTA > W3110S.gb/2893113‑2893242 | aGTACCAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCtt < 1:1139451/69‑1 (MQ=255) aCCAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTAttgtt < 1:3175989/69‑1 (MQ=255) tCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAAtt < 1:2846930/69‑1 (MQ=255) aGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCacaa > 1:2041376/1‑69 (MQ=255) aGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCacaa > 1:1643039/1‑69 (MQ=255) aGCGGCAAGAGCGGAAGGTTTTTTCTGTATTAATCGCCTGTTTATTGTTGATTCAATTTCGTTTCacaa > 1:3399216/1‑69 (MQ=255) gCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACAtt > 1:3519099/1‑69 (MQ=255) cAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTg < 1:3463831/69‑1 (MQ=255) aaGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGa < 1:3199650/69‑1 (MQ=255) aaGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGa < 1:1784726/69‑1 (MQ=255) agagCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGa < 1:460948/68‑1 (MQ=255) agCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATa > 1:690984/1‑68 (MQ=255) agCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATa > 1:1070587/1‑68 (MQ=255) agCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGt > 1:1737430/1‑53 (MQ=255) agCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGt > 1:300038/1‑53 (MQ=255) agCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGt > 1:1168545/1‑53 (MQ=255) agCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGt > 1:2545716/1‑53 (MQ=255) agCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGt > 1:2622860/1‑53 (MQ=255) cGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCtt < 1:2620151/35‑1 (MQ=255) ggAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCa < 1:2059055/69‑1 (MQ=255) ggAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCa < 1:162021/69‑1 (MQ=255) ggAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCa < 1:3610723/69‑1 (MQ=255) ggAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCa < 1:2525023/69‑1 (MQ=255) gAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCa < 1:1727057/68‑1 (MQ=255) gTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCa < 1:2788620/38‑1 (MQ=255) gTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCa < 1:2411560/38‑1 (MQ=255) tttttCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCg > 1:3691803/1‑42 (MQ=255) tttttCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCacaa > 1:858530/1‑50 (MQ=255) tttttCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCacaa > 1:1829893/1‑50 (MQ=255) tttCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCa > 1:2460422/1‑69 (MQ=255) tttCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCa > 1:3694046/1‑69 (MQ=255) tttCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCa > 1:1388199/1‑69 (MQ=255) ttCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTgcgc > 1:3298800/1‑60 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCg > 1:3686750/1‑69 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCg > 1:39514/1‑69 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCg > 1:3437023/1‑69 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCg > 1:3121132/1‑69 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCg > 1:1309547/1‑69 (MQ=255) cATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTg < 1:91050/43‑1 (MQ=255) cATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTg < 1:2189090/43‑1 (MQ=255) cATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTg < 1:1284781/43‑1 (MQ=255) cATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCg > 1:1579400/1‑69 (MQ=255) cTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGt > 1:3122004/1‑69 (MQ=255) cTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGt > 1:3094501/1‑69 (MQ=255) cTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGt > 1:2449660/1‑69 (MQ=255) cTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGt < 1:805730/69‑1 (MQ=255) tGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGt < 1:235491/68‑1 (MQ=255) tttCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTa > 1:1000065/1‑67 (MQ=255) tATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGGAATCGAATCCGGATTGTa > 1:2203078/1‑65 (MQ=255) tATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCa > 1:2076045/1‑48 (MQ=255) tATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTa > 1:2580120/1‑65 (MQ=255) tATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTa > 1:100451/1‑65 (MQ=255) tATTGTTGATTCAATTTCGTTT‑‑CAACATTGATAAGc < 1:2336330/36‑1 (MQ=37) | AGTACCAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTA > W3110S.gb/2893113‑2893242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |