| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,910,539 | C→A | 76.2% | R590L (CGC→CTC) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,910,539 | 0 | C | A | 76.2% | 20.9 / 13.3 | 21 | R590L (CGC→CTC) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base A (4/12); total (6/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTC > W3110S.gb/2910476‑2910591 | cgcgcgATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGttt < 1:3517137/69‑1 (MQ=255) cgATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTgcg < 1:2795228/69‑1 (MQ=255) cgATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTgcg < 1:2172507/69‑1 (MQ=255) gtgCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCgtg > 1:2110231/1‑67 (MQ=255) tCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCttt < 1:1714825/69‑1 (MQ=255) tCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCtt < 1:123509/68‑1 (MQ=255) tCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCtt < 1:2401343/68‑1 (MQ=255) cAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGa < 1:1611408/68‑1 (MQ=255) aaCACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTTCGGCGTGTAGCTTTTTTGCTGaa < 1:1107627/68‑1 (MQ=255) acCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTg > 1:3005959/1‑69 (MQ=255) cTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCt < 1:2233137/69‑1 (MQ=255) cTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCt < 1:1351593/69‑1 (MQ=255) cTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCt < 1:1331832/69‑1 (MQ=255) cTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCt < 1:695540/69‑1 (MQ=255) ttCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCttt < 1:2395220/53‑1 (MQ=255) aCCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCtt > 1:3083909/1‑45 (MQ=255) aCCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCGTTTTTGCTGAAGTTGCTTCAgcgc > 1:2600780/1‑68 (MQ=255) aCGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCgcggcg > 1:3392295/1‑69 (MQ=255) aCCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTc < 1:2592642/69‑1 (MQ=255) aTCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTc > 1:2813296/1‑49 (MQ=255) tttACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAg < 1:831459/48‑1 (MQ=255) | CGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTC > W3110S.gb/2910476‑2910591 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |