| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,931,037 | C→A | 42.7% | A212A (GCG→GCT) | fucO ← | L‑1,2‑propanediol oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,931,037 | 0 | C | A | 42.7% | 53.1 / 34.5 | 42 | A212A (GCG→GCT) | fucO | L‑1,2‑propanediol oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (8/16); new base A (10/8); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCAACCGATCCTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGC > W3110S.gb/2930971‑2931099 | aGCAACCGATCCTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAgcg > 1:591560/1‑68 (MQ=255) aCCGATCCTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCAc > 1:1594699/1‑69 (MQ=255) aCCGATCCTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCAc > 1:2930313/1‑69 (MQ=255) gATCCTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCAcgc > 1:85722/1‑68 (MQ=255) tCCTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGcc < 1:2882866/69‑1 (MQ=255) ccTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCa > 1:459576/1‑69 (MQ=255) gCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCAcg > 1:2084911/1‑60 (MQ=255) gcgcCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTaa < 1:3516863/69‑1 (MQ=255) gcgcCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTaa < 1:2724370/69‑1 (MQ=255) cgcCCCAGCAATGATTTCTATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTaa < 1:1952901/68‑1 (MQ=255) cgcCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTaa < 1:1188905/68‑1 (MQ=255) gcCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAata < 1:2733541/69‑1 (MQ=255) gcCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAata < 1:2784013/69‑1 (MQ=255) cccAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAAtata < 1:1898950/69‑1 (MQ=255) tGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAata < 1:1411923/59‑1 (MQ=255) tGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACgg > 1:1699058/1‑53 (MQ=255) aTTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTaa < 1:3057277/55‑1 (MQ=255) aTTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTaa < 1:3040376/55‑1 (MQ=255) aTTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTaa < 1:2959468/55‑1 (MQ=255) aTTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTaa < 1:3601739/55‑1 (MQ=255) aTTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAAt > 1:3146100/1‑68 (MQ=255) aTTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAAt > 1:1452102/1‑68 (MQ=255) aTTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAAt > 1:525965/1‑68 (MQ=255) cAATCGCTTTAATGTGCAGTGCATCGGTTAGAGccc > 1:2090179/1‑36 (MQ=255) cAATCGCTTTAATGTGCAGTGCATCGGTTAGAGccc > 1:1093452/1‑36 (MQ=255) cAATCGCTTTAATGTGCAGTGCATCGGTTAGAGccc > 1:2486390/1‑36 (MQ=255) tCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACgcgc < 1:1821991/39‑1 (MQ=255) tCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACgcgc < 1:2980484/39‑1 (MQ=255) gCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCAtgagtg > 1:1840548/1‑69 (MQ=255) tttAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAata < 1:3238426/46‑1 (MQ=255) aaTGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTaa < 1:674685/41‑1 (MQ=255) aaTGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTaa < 1:1133895/41‑1 (MQ=255) aaTGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTaa < 1:2625540/41‑1 (MQ=255) aaTGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTaa < 1:2248439/41‑1 (MQ=255) aTGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCa > 1:2395928/1‑69 (MQ=255) tgcagtgcaTCGGTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATc < 1:2403558/68‑1 (MQ=255) cagtgcaTCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGa < 1:1536258/68‑1 (MQ=255) gcaTCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACAccc > 1:2912833/1‑69 (MQ=255) gcaTCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACAccc < 1:53344/69‑1 (MQ=255) gcaTCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACAccc < 1:604085/69‑1 (MQ=255) gcaTCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATAGACAccc > 1:213360/1‑69 (MQ=255) ggTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGc > 1:2909490/1‑69 (MQ=255) ggTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGc > 1:369495/1‑69 (MQ=255) | AGCAACCGATCCTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGC > W3110S.gb/2930971‑2931099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |