| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,938,519 | A→G | 56.3% | I166V (ATC→GTC) | fucR → | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,938,519 | 0 | A | G | 56.3% | 30.6 / 90.6 | 71 | I166V (ATC→GTC) | fucR | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base A (9/22); new base G (27/13); total (36/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.83e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
CTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTCCA > W3110S.gb/2938456‑2938579 | cTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGAt > 1:3512731/1‑69 (MQ=255) ttGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATc > 1:444901/1‑69 (MQ=255) tGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATc > 1:673518/1‑68 (MQ=255) tGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATc > 1:3162249/1‑68 (MQ=255) tGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATc > 1:2329568/1‑68 (MQ=255) tGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATc > 1:1598580/1‑68 (MQ=255) gAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGa < 1:1674337/65‑1 (MQ=255) atatGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTAtttt < 1:1005505/69‑1 (MQ=255) atatGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTAtttt < 1:2759651/69‑1 (MQ=255) atatGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTAtttt < 1:1001216/69‑1 (MQ=255) gCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGa < 1:244582/52‑1 (MQ=255) tGTTAAGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTG‑TTATTTTTTCTtgtg < 1:1239265/69‑1 (MQ=255) gTTACGTTAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTtgtg < 1:2523290/69‑1 (MQ=255) gTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTtgtg < 1:1391275/69‑1 (MQ=255) gTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTtgtg < 1:3354327/69‑1 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGaa > 1:2489011/1‑68 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGaa > 1:2981021/1‑68 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGaa > 1:3263068/1‑68 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGa > 1:802670/1‑67 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCg < 1:3699424/45‑1 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCg < 1:2336285/45‑1 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCt < 1:2282208/62‑1 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCt < 1:2348262/62‑1 (MQ=255) cGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:1151290/1‑69 (MQ=255) tCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGa > 1:3049054/1‑69 (MQ=255) aaTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATc < 1:2917173/69‑1 (MQ=255) aaTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATc < 1:2442537/69‑1 (MQ=255) aaTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAg > 1:2381401/1‑64 (MQ=255) aaTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATc < 1:2567662/69‑1 (MQ=255) aaTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATc > 1:1216987/1‑69 (MQ=255) aaTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATc < 1:1644996/69‑1 (MQ=255) tCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCg < 1:2655486/68‑1 (MQ=255) cccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGAt < 1:1333951/69‑1 (MQ=255) cccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGAt < 1:469355/69‑1 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGAt > 1:447727/1‑68 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGAt > 1:2462818/1‑68 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTc > 1:503839/1‑52 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATc < 1:458503/65‑1 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATc > 1:1534792/1‑65 (MQ=255) tCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATagc < 1:1589239/69‑1 (MQ=255) gCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTtgtg < 1:162278/53‑1 (MQ=255) gCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTtgtg > 1:673295/1‑53 (MQ=255) gCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATagcag > 1:1652269/1‑69 (MQ=255) gCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATagcag > 1:2340991/1‑69 (MQ=255) ttCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATagc < 1:2857073/61‑1 (MQ=255) ttCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATagc < 1:172774/61‑1 (MQ=255) tCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATc > 1:3046229/1‑54 (MQ=255) tCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATc > 1:2002363/1‑54 (MQ=255) tCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATc > 1:2596209/1‑54 (MQ=255) tCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCa < 1:128093/69‑1 (MQ=255) tCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCa < 1:3118582/69‑1 (MQ=255) aaCTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACtgt > 1:1380851/1‑69 (MQ=255) aaCTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACtg > 1:3153993/1‑68 (MQ=255) cTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGa > 1:2012330/1‑50 (MQ=255) cTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGa < 1:877940/50‑1 (MQ=255) cTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGa < 1:2049395/50‑1 (MQ=255) cTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGa < 1:2225359/50‑1 (MQ=255) cTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGa > 1:273148/1‑50 (MQ=255) ttAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTggg > 1:1834263/1‑69 (MQ=255) tAAATCGCTGGAAGTCGATCTGTTTATTTTTTATTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGa > 1:3133074/1‑69 (MQ=255) tAAATCGCTGCAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGa > 1:483309/1‑69 (MQ=255) tAAATCGCAGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATa > 1:2113033/1‑50 (MQ=39) aaaTCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATc < 1:3507319/45‑1 (MQ=255) aaaTCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGAt > 1:1915826/1‑48 (MQ=255) aaaTCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGAt > 1:1701791/1‑48 (MQ=255) aaaTCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGAt > 1:2913760/1‑48 (MQ=255) tCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTcc < 1:908778/69‑1 (MQ=255) cGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATGGCAGCGGCGCACTGTGGGACTCCa > 1:684269/1‑69 (MQ=255) gAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTgg < 1:126567/57‑1 (MQ=255) gAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTgg < 1:1698956/57‑1 (MQ=255) gAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTgg < 1:1279055/57‑1 (MQ=255) | CTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTCCA > W3110S.gb/2938456‑2938579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |