| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,065,306 | A→G | 16.7% | V175A (GTT→GCT) | ygfI ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,065,306 | 0 | A | G | 16.7% | 127.7 / 19.3 | 54 | V175A (GTT→GCT) | ygfI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (27/18); new base G (6/3); total (33/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
TGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACT > W3110S.gb/3065246‑3065368 | tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAg > 1:1238816/1‑69 (MQ=255)tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAg > 1:3183787/1‑69 (MQ=255)tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAg > 1:1551718/1‑69 (MQ=255) cAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg > 1:2207959/1‑69 (MQ=255) cAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg > 1:2133740/1‑69 (MQ=255) cAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg > 1:3332464/1‑69 (MQ=255) tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:1249978/1‑68 (MQ=255) ccTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggt > 1:648174/1‑68 (MQ=255) ccTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggt > 1:28990/1‑68 (MQ=255) gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:2468280/68‑1 (MQ=255) gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:1750213/68‑1 (MQ=255) gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:485078/68‑1 (MQ=255) gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:3544371/68‑1 (MQ=255) gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:1007378/68‑1 (MQ=255) gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:11610/68‑1 (MQ=255) gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:368087/68‑1 (MQ=255) gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg < 1:667205/67‑1 (MQ=255) gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTa < 1:2673238/69‑1 (MQ=255) gcagcTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGtt < 1:3646194/69‑1 (MQ=255) cagcTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAg < 1:884751/66‑1 (MQ=255) agcTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaacgag < 1:3277954/41‑1 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg > 1:132270/1‑58 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg > 1:2255003/1‑58 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg > 1:2318337/1‑58 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg < 1:2462152/58‑1 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACt > 1:2157871/1‑67 (MQ=255) tgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa > 1:3725999/1‑69 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGtt > 1:817422/1‑60 (MQ=255) ctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:2964315/1‑65 (MQ=255) cttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCa < 1:366104/69‑1 (MQ=255) cttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGATCACTGATAGTCa < 1:3241188/69‑1 (MQ=255) ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggtggt < 1:1612633/52‑1 (MQ=255) gTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAg < 1:3377142/67‑1 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGtt > 1:2511401/1‑54 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGtt > 1:270081/1‑54 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:697120/1‑69 (MQ=255) ctctTCATAACGGCGAGAAACGAGAAAGCCTAATCGGAGCggtgg < 1:1930979/45‑1 (MQ=255) ctctTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg < 1:1261896/45‑1 (MQ=255) ctctTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg < 1:412116/45‑1 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACt < 1:2558953/49‑1 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACt > 1:1132690/1‑49 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACt > 1:3364848/1‑49 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACt < 1:1697260/49‑1 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc > 1:1303711/1‑65 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc > 1:2884506/1‑65 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc > 1:2163689/1‑65 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:588101/1‑69 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:99387/1‑69 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:3175273/1‑69 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:2425111/1‑69 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:1217974/1‑69 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGACAGGTTTTCCAGtt > 1:3283906/1‑69 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCACAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCAAGtt > 1:2148521/1‑69 (MQ=255) aCGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCa > 1:892583/1‑53 (MQ=255) aCGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCa > 1:1179627/1‑53 (MQ=255) cgagaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACt < 1:512981/69‑1 (MQ=255) cgagaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACt < 1:128712/69‑1 (MQ=255) agaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc < 1:1332734/54‑1 (MQ=255) acgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGt > 1:2641475/1‑54 (MQ=255) | TGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACT > W3110S.gb/3065246‑3065368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |