| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,079,041 | A→G | 52.7% | I417I (ATT→ATC) | tktA ← | transketolase 1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,079,041 | 0 | A | G | 52.7% | 13.2 / 104.1 | 74 | I417I (ATT→ATC) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base A (4/31); new base G (13/26); total (17/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
GAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTT > W3110S.gb/3078981‑3079103 | gAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGt < 1:2860679/69‑1 (MQ=255)gAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGt < 1:2987039/69‑1 (MQ=255)gAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGt < 1:2397150/69‑1 (MQ=255)gAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGt < 1:1559730/69‑1 (MQ=255)gAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGt < 1:2288662/69‑1 (MQ=255)gAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGt < 1:827682/69‑1 (MQ=255) aCATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCa > 1:1915930/1‑69 (MQ=255) tCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATa > 1:683102/1‑68 (MQ=255) tCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATa > 1:2660543/1‑68 (MQ=255) cAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCa > 1:3596798/1‑55 (MQ=255) cAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCa < 1:1438300/55‑1 (MQ=255) aGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCg < 1:2039798/69‑1 (MQ=255) aGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCg < 1:2207676/69‑1 (MQ=255) aGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCg < 1:3270699/69‑1 (MQ=255) gAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGGGGTCAt < 1:7385/63‑1 (MQ=255) gAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCAt < 1:2285301/63‑1 (MQ=255) gAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCAt < 1:1615546/63‑1 (MQ=255) gAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCAt < 1:3095404/63‑1 (MQ=255) gAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGa < 1:2346667/68‑1 (MQ=255) gAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGa < 1:3055278/68‑1 (MQ=255) gAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGa < 1:1088487/68‑1 (MQ=255) gAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTcgcg > 1:2706441/1‑68 (MQ=255) ggTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGaa < 1:705037/68‑1 (MQ=255) ggTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGaa < 1:3119091/68‑1 (MQ=255) gtACGGCAGGAATCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGaa < 1:2122055/65‑1 (MQ=255) gtACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAacac > 1:890847/1‑68 (MQ=255) gtACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGaa < 1:1314588/65‑1 (MQ=255) gtACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGaa < 1:563465/65‑1 (MQ=255) gtACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGaa < 1:3463285/65‑1 (MQ=255) gtACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGaa < 1:1007477/65‑1 (MQ=255) gtACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGaa < 1:117648/65‑1 (MQ=255) gtACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAacac > 1:2411367/1‑68 (MQ=255) tACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAacac > 1:1604002/1‑67 (MQ=255) tACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAacac > 1:3331942/1‑67 (MQ=255) tACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCg < 1:337183/69‑1 (MQ=255) tACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCg < 1:2925504/69‑1 (MQ=255) tACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCg < 1:1166133/69‑1 (MQ=255) tACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCg < 1:3494098/69‑1 (MQ=255) cGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGt < 1:275435/68‑1 (MQ=255) cGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTa > 1:1348741/1‑69 (MQ=255) gcagcaAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGt < 1:1322751/43‑1 (MQ=38) gCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGt < 1:905092/43‑1 (MQ=255) gCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGt < 1:1055450/43‑1 (MQ=255) gCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGt < 1:2799382/43‑1 (MQ=255) gCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATTCCGa < 1:3059765/51‑1 (MQ=255) cAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTg < 1:2990188/69‑1 (MQ=255) aGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:1711068/69‑1 (MQ=255) aGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:1542854/69‑1 (MQ=255) aGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:796458/69‑1 (MQ=255) aGCCACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGCGGATGt < 1:6550/69‑1 (MQ=255) aGCCACCGTGCACGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:922695/69‑1 (MQ=255) ccACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGa < 1:762442/43‑1 (MQ=255) ccACCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGa < 1:3309198/43‑1 (MQ=255) aCCGTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGtt > 1:878361/1‑69 (MQ=255) gTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTAcc > 1:1476807/1‑69 (MQ=255) gTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTAcc > 1:2370817/1‑69 (MQ=255) gTGCAGGGAGATACCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAATTAcc > 1:3100355/1‑69 (MQ=255) gggAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCgcag < 1:1296879/69‑1 (MQ=255) gggAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCgcag < 1:3111718/69‑1 (MQ=255) gggAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCgcag < 1:3420632/69‑1 (MQ=255) ggAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCgcagc < 1:305873/69‑1 (MQ=255) aTACCGTTAGCAATCGCGGTCATACCGAACTTGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCt < 1:2462235/69‑1 (MQ=255) aCCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTc > 1:401038/1‑69 (MQ=255) aCCGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTc > 1:3054851/1‑69 (MQ=255) aCCGTTAGCGAT‑‑CGGTCATACCGAACTCGCGAaca < 1:2506191/35‑1 (MQ=25) aCCGTTAGCAATCGCGGTCATACCGAACTCGCGAacac < 1:3185356/38‑1 (MQ=255) ccGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCgcagc > 1:2481249/1‑62 (MQ=255) ccGTTAGCGATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCg > 1:2862333/1‑69 (MQ=255) gTTAGCGATCGCGGTAATACAGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGtt < 1:1904425/69‑1 (MQ=255) gTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGtt < 1:3225746/51‑1 (MQ=255) gTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGtt < 1:462022/69‑1 (MQ=255) gTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGtt < 1:504808/69‑1 (MQ=255) gTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGtt < 1:2515048/69‑1 (MQ=255) gTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGtt < 1:1311489/69‑1 (MQ=255) gTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGtt < 1:297927/69‑1 (MQ=255) ttAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGtt < 1:3282235/50‑1 (MQ=255) | GAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTT > W3110S.gb/3078981‑3079103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |