| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 172,490 | A→G | 41.4% | E343E (GAA→GAG) | fhuB → | fused subunits of iron‑hydroxamate transporter and membrane components of ABC superfamily transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 172,490 | 0 | A | G | 41.4% | 10.4 / 33.3 | 29 | E343E (GAA→GAG) | fhuB | fused subunits of iron‑hydroxamate transporter and membrane components of ABC superfamily transporter |
| Reads supporting (aligned to +/- strand): ref base A (5/12); new base G (6/6); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTGTGGTGGT > W3110S.gb/172430‑172552 | gCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAACGCCaa < 1:3517687/67‑1 (MQ=255)gCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAACGCCaa < 1:2215455/67‑1 (MQ=255)gCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAACGCCAACa > 1:1567737/1‑69 (MQ=255)gCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAACGCCAACa > 1:189781/1‑69 (MQ=255) cagcaTTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGtt < 1:3647261/69‑1 (MQ=255) cagcaTTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGtt < 1:3202755/69‑1 (MQ=255) aTTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGcc > 1:1439426/1‑69 (MQ=255) cgcCGGATATTAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCgcgg < 1:362235/69‑1 (MQ=255) cgcCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCgcgg < 1:1067822/69‑1 (MQ=255) cgcCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCgcgg < 1:524215/69‑1 (MQ=255) cgcCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCgcgg < 1:2926675/69‑1 (MQ=255) tatGAAGGTCAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGt < 1:789326/69‑1 (MQ=255) tatGAAGGTCAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGt < 1:388154/69‑1 (MQ=255) gAAGGTCAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGggcggc > 1:2169157/1‑64 (MQ=255) cAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt > 1:186055/1‑69 (MQ=255) cAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt < 1:3169235/69‑1 (MQ=255) cAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt < 1:1786806/69‑1 (MQ=255) cAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt < 1:1524793/69‑1 (MQ=255) cAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt < 1:1322732/69‑1 (MQ=255) cAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt < 1:1305606/69‑1 (MQ=255) aaCGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGt < 1:2424863/40‑1 (MQ=255) aaCGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGt < 1:3720028/40‑1 (MQ=255) gATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGggcggc < 1:367441/54‑1 (MQ=255) gATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGtg > 1:2542230/1‑57 (MQ=255) cGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTg > 1:2455856/1‑37 (MQ=255) gcgGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTgtggtg > 1:2422622/1‑69 (MQ=255) gcgGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTgtggtg > 1:896382/1‑69 (MQ=255) gCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCgcgggc > 1:1199025/1‑39 (MQ=255) gCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTgtggtggt > 1:1372120/1‑68 (MQ=255) | GCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTGTGGTGGT > W3110S.gb/172430‑172552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |