| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,101,264 | G→A | 22.4% | A82V (GCT→GTT) | yggH ← | tRNA (m7G46) methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,101,264 | 0 | G | A | 22.4% | 101.8 / 23.8 | 49 | A82V (GCT→GTT) | yggH | tRNA (m7G46) methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (18/20); new base A (2/9); total (20/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTC > W3110S.gb/3101197‑3101311 | cAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGc < 1:2713934/69‑1 (MQ=255)cAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGc > 1:3005152/1‑69 (MQ=255)cAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGc < 1:2352448/69‑1 (MQ=255) aGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGcc < 1:83981/69‑1 (MQ=255) gcacCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGcc > 1:2204804/1‑63 (MQ=255) gcacCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGcc > 1:3334179/1‑63 (MQ=255) gcacCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGcc > 1:1876089/1‑63 (MQ=255) gcacCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGcc > 1:3535076/1‑63 (MQ=255) gcacCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGcc > 1:642359/1‑63 (MQ=255) aaCGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGccacca > 1:3401887/1‑68 (MQ=255) aaCGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGccacca > 1:72982/1‑68 (MQ=255) aaCGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGccacca > 1:1230249/1‑68 (MQ=255) aCGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGc < 1:652025/69‑1 (MQ=255) aCGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGc < 1:3166935/69‑1 (MQ=255) aCGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGc > 1:3000142/1‑69 (MQ=255) tGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCa < 1:1142398/50‑1 (MQ=255) tGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc < 1:906100/69‑1 (MQ=255) tGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc < 1:2456362/69‑1 (MQ=255) tGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc < 1:2447242/69‑1 (MQ=255) tGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc < 1:1236917/69‑1 (MQ=255) tGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc < 1:488154/69‑1 (MQ=255) cACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGc < 1:3226213/42‑1 (MQ=255) cACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGc > 1:1199944/1‑68 (MQ=255) cTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCaa < 1:215318/69‑1 (MQ=255) cTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCaa < 1:1588417/69‑1 (MQ=255) tCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGc < 1:2205772/38‑1 (MQ=255) tCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCaaa > 1:1699324/1‑68 (MQ=255) cAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGcc < 1:2414239/38‑1 (MQ=255) cAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGcc < 1:1021392/38‑1 (MQ=255) cAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGc < 1:3491422/37‑1 (MQ=255) cAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGccacca > 1:2176219/1‑48 (MQ=255) cAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCaaaa > 1:1312408/1‑68 (MQ=255) cAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCAACGCCCCCATGCCaaaa > 1:1729623/1‑68 (MQ=255) cAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCAACGCCCCCATGCCaaaa > 1:267832/1‑68 (MQ=255) tGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAAcc < 1:460609/67‑1 (MQ=255) tGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAACCa < 1:3401962/68‑1 (MQ=255) gcagAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAAt > 1:460895/4‑69 (MQ=255) gCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGc < 1:1786980/46‑1 (MQ=255) gCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAAt > 1:3492426/1‑69 (MQ=255) gCCGAGGAAGTCATGCTCAGGGCGATCTTTAGCCATTGCCGCCAGc < 1:2401588/46‑1 (MQ=38) gTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGt < 1:1886056/69‑1 (MQ=255) gTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGt < 1:1544965/69‑1 (MQ=255) gTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCACATGCCAAAACCAATCTCAAGCGt < 1:3075255/69‑1 (MQ=255) gTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAAcc > 1:1664300/1‑57 (MQ=255) ccTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTc < 1:1342778/68‑1 (MQ=255) cTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGc > 1:2376495/1‑61 (MQ=255) aTCTTTAACGATTGCCACCAGCGACGCCCCCATGCCAAAACCa < 1:1034202/43‑1 (MQ=38) aTCTTTAACCATTGCCACCAGCGACGCCCCCATGCCTAAACCa < 1:476235/43‑1 (MQ=38) aTCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAACCa < 1:1617564/43‑1 (MQ=255) | CAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTC > W3110S.gb/3101197‑3101311 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |