| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,125,971 | A→G | 50.0% | V236A (GTT→GCT) | glcD ← | glycolate oxidase subunit, FAD‑linked |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,125,971 | 0 | A | G | 50.0% | ‑4.0 / 34.7 | 26 | V236A (GTT→GCT) | glcD | glycolate oxidase subunit, FAD‑linked |
| Reads supporting (aligned to +/- strand): ref base A (4/9); new base G (3/10); total (7/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01 | |||||||||||
ATTGGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCC > W3110S.gb/3125907‑3126026 | aTTGGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCcg < 1:3372284/69‑1 (MQ=255)aTTGGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCcg < 1:3199738/69‑1 (MQ=255) ggCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAGCCCgcgc < 1:1237638/69‑1 (MQ=255) ggCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAGCCCgcgc > 1:302936/1‑69 (MQ=255) ggCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCgcgc > 1:1734925/1‑69 (MQ=255) ggCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCgcgc > 1:2205274/1‑69 (MQ=255) ggCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCgcgc > 1:3127879/1‑69 (MQ=255) tgatgTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACgg < 1:924249/69‑1 (MQ=255) gTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAGCCCGCGCCACGggcgg > 1:1551811/1‑69 (MQ=255) tCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGggcggc < 1:1435851/69‑1 (MQ=255) tCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGggcggc < 1:3519742/69‑1 (MQ=255) aaGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGcagcag < 1:95356/69‑1 (MQ=255) aaGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGcagcag < 1:707328/69‑1 (MQ=255) aaGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGcagcag < 1:541962/69‑1 (MQ=255) tCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAGCCCGCGCCACGGGCGGCTTCGGCAGCAGttt < 1:2949212/69‑1 (MQ=255) tCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGttt > 1:1203981/1‑69 (MQ=255) ccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAGCCCGCGCCACGGGCGGCTTCGGCAGCAGtttt < 1:3393518/69‑1 (MQ=255) gCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAGCCCGCGCCACGGGCGGCTTCGGCAGCAGtttt > 1:3330023/1‑66 (MQ=255) tttttCTACCGAGTCAAAGCTGGCTAACAGAGCCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGt < 1:291780/69‑1 (MQ=255) aCCGAGTCAAAGCTGGCTAACAGAGCCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTc < 1:1612893/68‑1 (MQ=255) aCCGAGTCAAAGCTGGCTAACAGAGCCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTc < 1:1292580/68‑1 (MQ=255) aCCGAGTCAAAGCTGGCTAACAGAGCCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTc < 1:1288383/68‑1 (MQ=255) aCCGAGTCAAAGCTGGCTAACAGAGCCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTc < 1:741478/68‑1 (MQ=255) gTCAAAGCTGGCTAACAGAGCCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCggtggt < 1:483299/69‑1 (MQ=255) aaaGCTGGCTAACAGAGCCCGCGCCACGGGCGGATTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCa < 1:1134117/68‑1 (MQ=255) gCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGcc < 1:889069/69‑1 (MQ=255) | ATTGGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCC > W3110S.gb/3125907‑3126026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |